...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Mutational analysis of Rox1, a DNA-bending repressor of hypoxic genes in Saccharomyces cerevisiae.
【24h】

Mutational analysis of Rox1, a DNA-bending repressor of hypoxic genes in Saccharomyces cerevisiae.

机译：Rox1突变分析，酿酒酵母中的低氧基因可弯曲的DNA阻遏物。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rox1 is a repressor of the hypoxic genes of Saccharomyces cerevisiae. It binds to a specific hypoxic consensus sequence in the upstream region of these genes and represses transcription in conjunction with the general repression complex Tup1-Ssn6. In this study, we demonstrated that the first 100 amino acids comprising the HMG domain of Rox1 were responsible for DNA binding and that when bound, Rox1 bent DNA at an angle of 90 degrees. A mutational analysis resulted in the isolation of seven missense mutations, all located within the HMG domain, that caused loss of DNA binding. The effect of these mutations on the structure of Rox1 was evaluated on the basis of the homology between Rox1 and the human male sex-determining protein SRY, for which a structural model is available. The failure to isolate missense mutations in the carboxy-terminal three-quarters of the protein prompted a deletion analysis of this region. The results suggested that this region was responsible for the repression function of Rox1 and that the repression information was redundant. This hypothesis was confirmed by using a set of fusions between sequences encoding the GAL4 DNA-binding domain and portions of ROX1. Those fusions containing either the entire carboxy-terminal region or either half of it were capable of repression. Repression by selected fusions was demonstrated to be dependent on Ssn6.
机译：Rox1是酿酒酵母低氧基因的阻遏物。它与这些基因上游区域的特定缺氧共有序列结合，并与一般的抑制复合体Tup1-Ssn6一起抑制转录。在这项研究中，我们证明了包含Rox1的HMG域的前100个氨基酸负责DNA的结合，并且结合后，Rox1将DNA弯曲90度。突变分析导致分离出七个均位于HMG域内的错义突变，这些错义突变导致DNA结合丧失。根据Rox1与人类性别决定蛋白SRY之间的同源性，评估了这些突变对Rox1结构的影响，对此结构模型可用。无法在蛋白质的羧基末端四分之三处分离错义突变，导致对该区域的缺失分析。结果表明该区域负责Rox1的抑制功能，并且抑制信息是多余的。通过使用一组编码GAL4 DNA结合结构域的序列与ROX1的部分之间的融合，证实了这一假设。那些包含整个羧基末端区域或其中一半的融合体都能被抑制。证明了通过选择的融合的阻遏依赖于Ssn6。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号