A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes.

A K Lofquist; A D Garcia; S J Sharp

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A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes.

机译：在起始位点上游以22个碱基对为中心的离散区域调节果蝇tRNAAsn基因的转录。

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We have studied the mechanism by which 5'-flanking sequences modulate the in vitro transcription of eucaryotic tRNA genes. Using deletion and linker substitution mutagenesis, we have found that the 5'-flanking sequences responsible for the different in vitro transcription levels of three Drosophila tRNA5Asn genes are contained within a discrete region centered 22 nucleotides upstream from the transcription initiation site. In conjunction with the A-box intragenic control region, this upstream transcription-modulatory region functions in the selection mechanism for the site of transcription initiation. Since the transcription-modulatory region directs the position of the start site and the actual sequence of the transcription-modulatory region determines the level of tRNAAsn gene transcription, the possibility is raised that the transcription-modulatory region directs a transcription initiation event similar to open complex formation at procaryotic promoters.

机译：我们已经研究了5'侧翼序列调节真核tRNA基因体外转录的机制。使用缺失和接头取代诱变，我们发现负责3个果蝇tRNA5Asn基因体外转录水平不同的5'侧翼序列包含在以转录起始位点为上游22个核苷酸的离散区域内。结合A-box基因内控制区，该上游转录调节区在转录起始位点的选择机制中起作用。由于转录调节区指导起始位点的位置，而转录调节区的实际序列决定了tRNAAsn基因转录的水平，因此提高了转录调节区指导与起始复合物类似的转录起始事件的可能性。原核启动子形成。

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《Molecular and Cellular Biology》 |1988年第10期|共9页
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A K Lofquist; A D Garcia; S J Sharp;
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中图分类细胞生物学;
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6. A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes. [O] . A K Lofquist, A D Garcia, S J Sharp 1988

机译：在起始位点上游以22个碱基对为中心的离散区域调节果蝇tRNAAsn基因的转录。
7. A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes. [O] . Lofquist, A K, Garcia, A D, Sharp, S J 1988

机译：在起始位点上游以22个碱基对为中心的离散区域调节果蝇tRNAAsn基因的转录。
8. RNA Polymerase-Binding and Transcription Initiation Sites Upstream of the Methyl Reductase Operon of 'Methanococcus vannielii' [R] . Thomm, M., Sherf, B. A., Reeve, J. N. 1988

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A discrete region centered 22 base pairs upstream of the initiation site modulates transcription of Drosophila tRNAAsn genes.

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