首页> 外文期刊>Molecular and Cellular Biology >Transcription analysis of class II human leukocyte antigen genes from normal and immunodeficient B lymphocytes, using polymerase chain reaction.
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Transcription analysis of class II human leukocyte antigen genes from normal and immunodeficient B lymphocytes, using polymerase chain reaction.

机译:使用聚合酶链反应,从正常和免疫缺陷的B淋巴细胞中进行II类人类白细胞抗原基因的转录分析。

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摘要

The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.
机译:通过cDNA的聚合酶链反应扩增从II类先天性免疫缺陷B细胞确定所有人类II类白细胞抗原基因座的RNA转录水平。在可以观察到0.01%正常水平的条件下没有观察到mRNA。 Pre-mRNA可以从正常B细胞​​中扩增而不能从免疫缺陷B细胞中扩增,表明转录缺陷。

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