• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Nucleosome assembly of simian virus 40 DNA in a mammalian cell extract.
【24h】

Nucleosome assembly of simian virus 40 DNA in a mammalian cell extract.

机译：猿猴病毒40 DNA在哺乳动物细胞提取物中的核小体装配。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report here a mammalian cell-free system that can support chromatin assembly. Effective nucleosome assembly in HeLa cell extracts occurred at 125 to 200 mM KCl or potassium glutamate. At this physiological K+ ion concentration, two types of chromatin assembly were observed. The first was interfered with by Mg2+. Other cations such as Mn2+, Ca2+, Fe3+, and spermidine also inhibited this type of nucleosome assembly. The second type of assembly occurred in the presence of Mg2+ and at least equimolar ATP. However, even in the presence of ATP, excess Mg2+ inhibited assembly and promoted catenation of DNA; these effects could be circumvented by excess ATP, GTP, EDTA, or polyglutamic acid. The critical DNA concentration for optimum assembly in both pathways suggested a stoichiometric association of histones with DNA. The spacing of nucleosomes formed by both types of assembly on linear and circular DNA was reasonably regular, but chromatin assembled in the presence of ATP and Mg2+ was more stable.
机译：我们在这里报告可以支持染色质组装的哺乳动物无细胞系统。 HeLa细胞提取物中的有效核小体组装在125至200 mM KCl或谷氨酸钾时发生。在此生理K +离子浓度下，观察到两种类型的染色质组装体。第一个受到Mg2 +的干扰。其他阳离子，例如Mn2 +，Ca2 +，Fe3 +和亚精胺也抑制这种类型的核小体组装。第二类组装发生在Mg2 +和至少等摩尔ATP的存在下。但是，即使存在ATP，过量的Mg2 +也会抑制组装并促进DNA的串联。这些影响可以通过过量的ATP，GTP，EDTA或聚谷氨酸来避免。两种途径中最佳组装的临界DNA浓度表明组蛋白与DNA的化学计量关系。由线性和环状DNA上的两种组装类型形成的核小体的间隔是合理规则的，但是在ATP和Mg2 +存在下组装的染色质更稳定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号