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首页> 外文期刊>Molecular and Cellular Biology >RFX1 is identical to enhancer factor C and functions as a transactivator of the hepatitis B virus enhancer.
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RFX1 is identical to enhancer factor C and functions as a transactivator of the hepatitis B virus enhancer.

机译：RFX1与增强因子C相同，并充当乙肝病毒增强剂的反式激活剂。

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Hepatitis B virus gene expression is to a large extent under the control of enhancer I (EnhI). The activity of EnhI is strictly dependent on the enhancer factor C (EF-C) site, an inverted repeat that is bound by a ubiquitous nuclear protein known as EF-C. Here we report the unexpected finding that EF-C is in fact identical to RFX1, a novel transcription factor previously cloned by virtue of its affinity for the HLA class II X-box promoter element. This finding has allowed us to provide direct evidence that RFX1 (EF-C) is crucial for EnhI function in HepG2 hepatoma cells; RFX1-specific antisense oligonucleotides appear to inhibit EnhI-driven expression of the hepatitis B virus major surface antigen gene, and in transfection assays, RFX1 behaves as a potent transactivator of EnhI. Interestingly, transactivation of EnhI by RFX1 (EF-C) is not observed in cell lines that are not of liver origin, suggesting that the ubiquitous RFX1 protein cooperates with liver-specific factors.
机译：乙型肝炎病毒基因表达在很大程度上受增强子I（EnhI）的控制。 EnhI的活性严格取决于增强因子C（EF-C）位点，该位点是一个反向重复序列，与被称为EF-C的普遍存在的核蛋白结合。在这里，我们报告了一个出乎意料的发现，即EF-C实际上与RFX1相同，RFX1是一种先前因其对HLA II类X-box启动子元件的亲和力而克隆的新型转录因子。这一发现使我们能够提供直接证据，证明RFX1（EF-C）对于HepG2肝癌细胞中EnhI功能至关重要。 RFX1特异性反义寡核苷酸似乎可以抑制EnhI驱动的乙型肝炎病毒主要表面抗原基因的表达，在转染测定中，RFX1充当EnhI的有效反式激活因子。有趣的是，在非肝脏起源的细胞系中未观察到RFX1（EF-C）对EnhI的反式激活，这表明普遍存在的RFX1蛋白与肝脏特异性因子协同作用。

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