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首页> 外文期刊>Molecular and Cellular Biology >Identification of I-plastin, a human fimbrin isoform expressed in intestine and kidney.
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Identification of I-plastin, a human fimbrin isoform expressed in intestine and kidney.

机译：鉴定I-plastin，一种在肠道和肾脏中表达的人纤维蛋白同工型。

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The complete cDNA sequence of human intestine-specific plastin (I-plastin) was determined from a clone derived by PCR. It consists of a 97-bp 5' untranslated region, a 1,887-bp coding region, and a 1,655-bp 3' untranslated region. The coding region predicts a 629-residue polypeptide whose sequence displays 86, 75, and 73% identities with chicken intestine fimbrin, human T-plastin, and human L-plastin, respectively. Recombinant I-plastin cross-linked actin filaments into bundles in the absence but not in the presence of calcium. The I-plastin gene was mapped by PCR to human chromosome 3; the L- and T-plastin genes were previously mapped to chromosomes 13 and X, respectively. I-plastin mRNA was detected in the small intestine, colon, and kidneys; relatively lower levels of expression were detected in the lungs and stomach. In contrast, L-plastin expression was restricted to the spleen and other lymph node-containing organs, while T-plastin was expressed in a variety of organs, including muscle, brain, uterus, and esophagus. In contrast to the situation for the intestine, high levels of L- and T-plastin mRNAs were detected in Caco-2, a human colon-derived cell line. Immunofluorescence microscopy detected I-plastin in the brush border of the small intestine and colon. These results identify I-plastin as the human homolog of chicken intestine fimbrin and as a third plastin isoform in humans.
机译：从通过PCR获得的克隆中确定人肠特异性增塑素（I-plastin）的完整cDNA序列。它由97bp的5'非翻译区，1887bp的编码区和1655bp的3'非翻译区组成。编码区可预测一个629个残基的多肽，其序列分别与鸡肠菌丝蛋白，人T-增塑素和人L-增塑素显示86％，75％和73％的同一性。在不存在钙但不存在钙的情况下，重组I-plastin将肌动蛋白丝交联成束。通过PCR将I-plastin基因定位到人类3号染色体上。 L-和T-plastin基因先前分别定位于13号和X号染色体。在小肠，结肠和肾脏中检测到了I-plastin mRNA。在肺和胃中检测到相对较低的表达水平。相比之下，L-plastin表达仅限于脾脏和其他含有淋巴结的器官，而T-plastin表达于多种器官，包括肌肉，脑，子宫和食道。与肠道情况相反，在人类结肠来源的细胞系Caco-2中检测到高水平的L-和T-plastin mRNA。免疫荧光显微镜检查在小肠和结肠的刷状缘中检测到了I-plastin。这些结果将I-增塑蛋白鉴定为鸡肠纤维蛋白的人同源物，并且是人中的第三种增塑蛋白同工型。

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