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首页> 外文期刊>Molecular and Cellular Biology >HPR1 encodes a global positive regulator of transcription in Saccharomyces cerevisiae.
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HPR1 encodes a global positive regulator of transcription in Saccharomyces cerevisiae.

机译：HPR1编码酿酒酵母中的转录的整体正调控器。

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摘要

The Hpr1 protein has an unknown function, although it contains a region of homology to DNA topoisomerase I. We have found that hpr1 null mutants are defective in the transcription of many physiologically unrelated genes, including GAL1, HO, ADH1, and SUC2, by using a combination of Northern (RNA) blot analysis, primer extension, and upstream activation sequence-lacZ fusions. Many of the genes positively regulated by HPR1 also require SWI1, SWI2-SNF2, SWI3, SNF5, and SNF6. The transcriptional defect at HO and the CCB::lacZ upstream activation sequence in hpr1 mutants is partially suppressed by a deletion of SIN1, which encodes an HMG1p-like protein. Elevated gene dosage of either histones H3 and H4 or H2A and H2B results in a severe growth defect in combination with an hpr1 null mutation. However, increased gene dosage of all four histones simultaneously restores near-normal growth in hpr1 mutants. Altered in vivo Dam methylase sensitivity is observed at two HPR1-dependent promoters (GAL1 and SUC2). Most of the Hpr1 protein present in the cell is in a large complex (10(6) Da) that is distinct from the SWI-SNF protein complex. We propose that HPR1 affects transcription and recombination by altering chromatin structure.
机译：Hpr1蛋白具有未知功能，尽管它包含与DNA拓扑异构酶I的同源区域。 Northern（RNA）印迹分析，引物延伸和上游激活序列-lacZ融合的组合。 HPR1积极调控的许多基因也需要SWI1，SWI2-SNF2，SWI3，SNF5和SNF6。 hpr1突变体中HO和CCB :: lacZ上游激活序列的转录缺陷被SIN1缺失部分抑制，该SIN1编码HMG1p样蛋白。组蛋白H3和H4或H2A和H2B的基因剂量升高会导致严重的生长缺陷，并伴有hpr1 null突变。但是，增加所有四个组蛋白的基因剂量可同时恢复hpr1突变体中接近正常的生长。在两个依赖HPR1的启动子（GAL1和SUC2）上观察到了体内Dam甲基化酶敏感性的改变。细胞中存在的大多数Hpr1蛋白都位于一个不同于SWI-SNF蛋白复合物的大复合物（10（6）Da）中。我们建议HPR1通过改变染色质结构影响转录和重组。

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