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首页> 外文期刊>Molecular and Cellular Biology >Quail Pax-6 (Pax-QNR) mRNAs are expressed from two promoters used differentially during retina development and neuronal differentiation.
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Quail Pax-6 (Pax-QNR) mRNAs are expressed from two promoters used differentially during retina development and neuronal differentiation.

机译：鹌鹑Pax-6（Pax-QNR）mRNA从在视网膜发育和神经元分化过程中差异使用的两个启动子表达。

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摘要

During investigations on the regulation of the Pax-6 gene, we characterized a cDNA from quail neuroretina showing a 5' untranslated region distinct from that previously described and initiated from an internal promoter. Using RNase protection and primer extension mapping, we localized this second quail Pax-6 promoter, termed P1. As reported for the already described P0 promoter, P1 was also transactivated in vitro by the p46Pax-QNR protein. RNase protection assays performed with quail neuroretina RNA showed that P1-initiated mRNAs were detected before the P0-initiated mRNAs, remained constant up to embryonic day 8, and decreased slowly thereafter whereas, P0-initiated mRNAs accumulated up to embryonic day 8. In contrast, quail retinal pigmented epithelium expressed only the P1-initiated mRNAs. Transformation of these cells by the v-myc oncogene induced neuronal traits in the culture, which thereafter, in addition to the P1-initiated mRNAs, expressed Pax-QNR from the P0 promoter. These results suggest that expression of the quail Pax-6 gene is under the control of different regulators through alternate promoters, P0 being activated at the onset of neuronal differentiation.
机译：在研究Pax-6基因的调控过程中，我们从鹌鹑神经视网膜中提取了一个cDNA，显示了一个5'非翻译区，该区与先前描述的区不同，并从内部启动子启动。使用RNase保护和引物延伸图谱，我们定位了第二个鹌鹑Pax-6启动子，称为P1。如已经描述的P0启动子报道的那样，P1在体外也被p46Pax-QNR蛋白反式激活。用鹌鹑神经视网膜RNA进行的RNase保护试验表明，在P0起始的mRNA之前检测到P1起始的mRNA，直到胚胎第8天保持恒定，然后缓慢下降，而P0起始的mRNA一直积累到胚胎第8天。 ，鹌鹑视网膜色素上皮仅表达P1启动的mRNA。 v-myc致癌基因对这些细胞的转化诱导了培养物中的神经元性状，此后除了P1启动的mRNA外，还从P0启动子表达了Pax-QNR。这些结果表明，鹌鹑Pax-6基因的表达受交替启动子控制在不同的调节子的控制下，P0在神经元分化开始时被激活。

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