Rapamycin Induces the G0 Program of Transcriptional Repression in Yeast by Interfering with the TOR Signaling Pathway

Dean Zaragoza; Ataollah Ghavidel; Joseph Heitman; Michael C. Schultz

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Rapamycin Induces the G0 Program of Transcriptional Repression in Yeast by Interfering with the TOR Signaling Pathway

机译：雷帕霉素通过干扰TOR信号转导途径诱导酵母转录抑制的G0程序

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The macrolide antibiotic rapamycin inhibits cellular proliferation by interfering with the highly conserved TOR (for target of rapamycin) signaling pathway. Growth arrest of budding yeast cells treated with rapamycin is followed by the program of molecular events that characterizes entry into G₀ (stationary phase), including the induction of polymerase (Pol) II genes typically expressed only in G₀. Normally, progression into G₀ is characterized by transcriptional repression of the Pol I and III genes. Here, we show that rapamycin treatment also causes the transcriptional repression of Pol I and III genes. The down-regulation of Pol III transcription is TOR dependent. While it coincides with translational repression by rapamycin, transcriptional repression is due in part to a translation-independent effect that is evident in extracts from a conditional tor2 mutant. Biochemical experiments reveal that RNA Pol III and probably transcription initiation factor TFIIIB are targets of repression by rapamycin. In view of previous evidence that TFIIIB and Pol III are inhibited when protein phosphatase 2A (PP2A) function is impaired, and that PP2A is a component of the TOR pathway, our results suggest that TOR signaling regulates Pol I and Pol III transcription in response to nutrient growth signals.

机译：大环内酯类抗生素雷帕霉素通过干扰高度保守的TOR（雷帕霉素的靶标）信号传导途径来抑制细胞增殖。用雷帕霉素处理的出芽酵母细胞的生长停滞之后是表征进入G _{0 （静止期）的分子事件程序，包括诱导通常仅在G中表达的聚合酶（Pol）II基因_{0 。通常，进展为G _{0 的特征在于Pol I和III基因的转录抑制。在这里，我们显示雷帕霉素治疗还引起Pol I和III基因的转录抑制。 Pol III转录的下调与TOR有关。虽然它与雷帕霉素的翻译抑制相吻合，但转录抑制部分归因于翻译非依赖性作用，这在条件 tor2 突变体的提取物中很明显。生化实验表明，RNA Pol III和可能的转录起始因子TFIIIB是雷帕霉素抑制的靶标。鉴于以前的证据表明，当蛋白磷酸酶2A（PP2A）功能受损时TFIIIB和Pol III会被抑制，并且PP2A是TOR途径的组成部分，我们的结果表明TOR信号传导响应于营养增长信号。}}} 展开▼

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《Molecular and Cellular Biology》 |1998年第8期|共8页

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Dean Zaragoza; Ataollah Ghavidel; Joseph Heitman; Michael C. Schultz;
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中图分类细胞生物学;

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专利

1. Target of rapamycin (TOR) signaling coordinatestRNA and 5S rRNA gene transcription with growthrate in yeast [J] . Dr. Michael C. Schultz Gene Therapy and Molecular Biology . 1999,第4aSuppla2期

机译：雷帕霉素（TOR）信号转导靶标tRNA和5S rRNA基因在酵母中的生长速率

2. Pmr1, a golgi Ca2+/Mn2+-ATPase, is a regulator of the target of rapamycin (TOR) signaling pathway in yeast [J] . Devasahayam G, Ritz D, Helliwell SB, Proceedings of the National Academy of Sciences of the United States of America . 2006,第47期

机译：Pmr1是一种高尔基Ca2 + / Mn2 + -ATPase，是酵母中雷帕霉素（TOR）信号转导靶标的调节剂

3. Partitioning the transcriptional program induced by rapamycin among the effecters of the Tor proteins [J] . Shamji AF., Schreiber SL., Kuruvilla FG. Current Biology: CB . 2000,第24期

机译：将雷帕霉素诱导的转录程序划分为Tor蛋白的效应子。

4. Increasing supply of Met and Arg relative to Lys while maintaining ratios of Thr∶Phe,Lys∶Thr, Lys∶His, and Lys∶Val alters casein protein abundance via AA-induced upstream mammalian target of rapamycin signaling pathway and circadian clock of bovine mammary epithelial cells [C] . L.Y.Hu, Y.F.Chen, I.M.Cortes, The 6th International Symposium on Dairy Cow Nutrition and Milk Quality（第六届“奶牛营养与牛奶质量”国际研讨会）论文集 . -1

机译：越来越多的遇见和arg的供应，而相对于李斯，而且保持鼠窜，lys:tr，lys:his和lys:val通过AA诱导的雷帕霉素信号传导途径和牛乳清昼夜昼夜昼夜昼夜急性的哺乳动物，改变酪蛋白蛋白质丰富上皮细胞

5. A Global Kinase and Phosphatase Interaction Network in the Budding Yeast Reveals Novel Effectors of the Target of Rapamycin (TOR) Pathway. [D] . Sharom, Jeffrey Roslan. 2011

机译：萌芽酵母中的全球激酶和磷酸酶相互作用网络揭示了雷帕霉素（TOR）途径的目标的新型效应子。

6. Rapamycin Induces the G0 Program of Transcriptional Repression in Yeast by Interfering with the TOR Signaling Pathway [O] . Dean Zaragoza, Ataollah Ghavidel, Joseph Heitman, 1998

机译：雷帕霉素通过干扰TOR信号通路诱导酵母中的G0程序转录抑制。

7. Rapamycin Induces the G0 Program of Transcriptional Repression in Yeast by Interfering with the TOR Signaling Pathway [O] . Zaragoza, Dean, Ghavidel, Ataollah, Heitman, Joseph, 1998

机译：雷帕霉素通过干扰TOR信号通路诱导酵母中的G0程序转录抑制。

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Rapamycin Induces the G0 Program of Transcriptional Repression in Yeast by Interfering with the TOR Signaling Pathway

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