• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Sir Proteins, Rif Proteins, and Cdc13p BindSaccharomyces Telomeres In Vivo
【24h】

Sir Proteins, Rif Proteins, and Cdc13p BindSaccharomyces Telomeres In Vivo

机译：Sir蛋白，Rif蛋白和Cdc13p体内结合酿酒酵母端粒

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although a surprisingly large number of genes affect yeast telomeres, in most cases it is not known if their products act directly or indirectly. We describe a one-hybrid assay for telomere binding proteins and use it to establish that six proteins that affect telomere structure or function but which had not been shown previously to bind telomeres in vivo are indeed telomere binding proteins. A promoter-defective allele of HIS3 was placed adjacent to a chromosomal telomere. Candidate proteins fused to a transcriptional activation domain were tested for the ability to activate transcription of the telomere-linked HIS3 gene. Using this system, Rif1p, Rif2p, Sir2p, Sir3p, Sir4p, and Cdc13p were found to be in vivo telomere binding proteins. None of the proteins activated the same reporter gene when it was at an internal site on the chromosome. Moreover, Cdc13p did not activate the reporter gene when it was adjacent to an internal tract of telomeric sequence, indicating that Cdc13p binding was telomere limited in vivo. The amino-terminal 20% of Cdc13p was sufficient to target Cdc13p to a telomere, suggesting that its DNA binding domain was within this portion of the protein. Rap1p, Rif1p, Rif2p, Sir4p, and Cdc13p activated the telomeric reporter gene in a strain lacking Sir3p, which is essential for telomere position effect (TPE). Thus, the telomeric association of these proteins did not require any of the chromatin features necessary for TPE. The data support models in which the telomere acts as an initiation site for TPE by recruiting silencing proteins to the chromosome end.
机译：尽管数量惊人的基因影响酵母端粒，但在大多数情况下，尚不清楚它们的产物是直接还是间接起作用。我们描述了端粒结合蛋白的一种杂交测定法，并使用它来确定影响端粒结构或功能但先前尚未显示出在体内结合端粒的六种蛋白确实是端粒结合蛋白。 HIS3 的启动子缺陷等位基因与染色体端粒相邻。测试与转录激活结构域融合的候选蛋白激活端粒连接的 HIS3 基因转录的能力。使用此系统，发现Rif1p，Rif2p，Sir2p，Sir3p，Sir4p和Cdc13p是体内端粒结合蛋白。当它们位于染色体的内部位点时，没有一种蛋白质激活相同的报告基因。此外，当Cdc13p与端粒序列的内部区域相邻时，它不会激活报告基因，这表明Cdc13p的结合在体内是端粒受限的。 Cdc13p的氨基末端20％足以将Cdc13p靶向端粒，表明其DNA结合结构域在蛋白质的这一部分内。 Rap1p，Rif1p，Rif2p，Sir4p和Cdc13p在缺少Sir3p的菌株中激活了端粒报道基因，这对于端粒位置效应（TPE）是必不可少的。因此，这些蛋白质的端粒缔合不需要TPE所需的任何染色质特征。数据支持模型，其中端粒通过将沉默蛋白募集到染色体末端而充当TPE的起始位点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号