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首页> 外文期刊>Molecular and Cellular Biology >The proto-oncogene c-ets is preferentially expressed in lymphoid cells.
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The proto-oncogene c-ets is preferentially expressed in lymphoid cells.

机译：原癌基因c-ets优先在淋巴样细胞中表达。

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The transforming sequences of the avian acute leukemia virus, E26, contain two distinct oncogenes, v-mybE and v-ets, fused together. By using a probe containing v-ets sequences, polyadenylated transcripts of the c-ets proto-oncogene were detected in avian tissues; they included a major 7.0-kilobase and a minor 2.0-kilobase species. These c-ets mRNAs were detected at high levels only in lymphoid organs and in avian T and B lymphoid cell lines. A similar pattern of c-ets transcription was observed in human hematopoietic cell lines, with transcripts detected in lymphoid B and T cells but not in erythroid or myeloid cells. The E26 oncogene was inserted into an inducible expression vector, and a 90-kilodalton protein (bp90) was produced in bacteria. Rabbit antisera raised to purified bp90 precipitated P135gag-mybE-ets, the v-mybE-ets polyprotein expressed in E26-transformed cells, and also reacted with p50v-mybA, the transforming protein of the avian myeloblastosis virus. Antiserum to bp90 was absorbed with a bacterially synthesized v-mybA protein to remove anti-myb activity. The absorbed anti-bp90 serum retained the ability to immunoprecipitate P135gag-mybE-ets from E26-transformed cells and specifically reacted with a 56-kilodalton polypeptide (p56) detected in chicken lymphoid organs and in T and B lymphocytes of both avian and human origin. The data suggest that p56 is a translational product of the c-ets proto-oncogene and imply that p56 may be involved in regulating the growth of lymphoid cells.
机译：禽急性白血病病毒E26的转化序列包含两个不同的癌基因，v-mybE和v-ets，融合在一起。通过使用含有v-ets序列的探针，在禽类组织中检测到c-ets原癌基因的聚腺苷酸转录本。他们包括一个主要的7.0千碱基和一个次要的2.0千碱基种。这些c-ets mRNA仅在淋巴器官以及禽T和B淋巴细胞系中高水平检测到。在人类造血细胞系中观察到了类似的c-ets转录模式，在淋巴B和T细胞中检出了转录本，而在红系或髓样细胞中未检出。将E26癌基因插入诱导型表达载体中，并在细菌中产生90-千达尔顿蛋白（bp90）。兔抗血清升至纯化的bp90会沉淀P135gag-mybE-ets（在E26转化的细胞中表达的v-mybE-ets多蛋白），并与禽成纤维细胞病病毒的转化蛋白p50v-mybA反应。 bp90的抗血清被细菌合成的v-mybA蛋白吸收，以去除抗myb活性。吸收的抗bp90血清保留了从E26转化的细胞中免疫沉淀P135gag-mybE-ets的能力，并与在鸡淋巴器官以及禽和人来源的T和B淋巴细胞中检测到的56千达尔顿多肽（p56）特异性反应。数据表明p56是c-ets原癌基因的翻译产物，并且暗示p56可能参与调节淋巴样细胞的生长。

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