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首页> 外文期刊>Molecular and Cellular Biology >TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins.
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TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins.

机译：TAPA-1是抗增殖抗体的靶标，它定义了一个新的跨膜蛋白家族。

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摘要

A murine monoclonal antibody was identified by its ability to induce a reversible antiproliferative effect on a human lymphoma cell line. Immunoprecipitation studies revealed that the antibody reacted with a 26-kilodalton cell surface protein (TAPA-1). A diverse group of human cell lines, including hematolymphoid, neuroectodermal, and mesenchymal cells, expressed the TAPA-1 protein. Many of the lymphoid cell lines, in particular those derived from large cell lymphomas, were susceptible to the antiproliferative effects of the antibody. TAPA-1 may therefore play an important role in the regulation of lymphoma cell growth. A cDNA clone coding for TAPA-1 was isolated by using the monoclonal antibody to screen an expression library in COS cells. Analysis of the deduced amino acid sequence indicated that the protein is highly hydrophobic and that it contains four putative transmembrane domains and a potential N-myristoylation site. TAPA-1 showed strong homology with the CD37 leukocyte antigen and with the ME491 melanoma-associated antigen, both of which have been implicated in the regulation of cell growth.
机译：通过其对人淋巴瘤细胞系诱导可逆的抗增殖作用的能力来鉴定鼠单克隆抗体。免疫沉淀研究表明，该抗体与26千达尔顿细胞表面蛋白（TAPA-1）反应。包括血淋巴样，神经外胚层和间充质细胞在内的多种人类细胞系表达了TAPA-1蛋白。许多淋巴样细胞系，特别是衍生自大细胞淋巴瘤的那些，对抗体的抗增殖作用敏感。因此，TAPA-1在调节淋巴瘤细胞生长中可能起重要作用。通过使用单克隆抗体筛选COS细胞中的表达文库，分离出编码TAPA-1的cDNA克隆。对推导的氨基酸序列的分析表明该蛋白是高度疏水的，并且它包含四个推定的跨膜结构域和一个潜在的N-肉豆蔻酰化位点。 TAPA-1与CD37白细胞抗原和与ME491黑色素瘤相关的抗原显示出很强的同源性，这两者均与细胞生长的调节有关。

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