High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.

K R Thomas; C Deng; M R Capecchi

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High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.

机译：通过使用序列替换载体将高保真基因靶向胚胎干细胞。

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Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides by several kilobases of DNA homologous to the target locus, replacement of chromosomal sequences with the exogenous DNA occurred with precision. If, on the other hand, the target-homologous DNA on one arm of the vector was reduced to below 1 kb in length, the fidelity of recombination was diminished.

机译：通过使用序列替换载体，将突变靶向鼠胚胎干细胞中的Hprt基因座。当设计载体以使突变序列的两侧侧接与靶基因座同源的几千个碱基的DNA时，精确地发生了用外源DNA置换染色体序列。另一方面，如果载体的一个臂上的靶同源DNA的长度减少到1kb以下，则重组的保真度降低。

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《Molecular and Cellular Biology》 |1992年第7期|共5页
作者
K R Thomas; C Deng; M R Capecchi;
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中图分类细胞生物学;
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1. Retraction Note: Targeted suicide gene therapy for glioma using human embryonic stem cell-derived neural stem cells genetically modified by baculoviral vectors. [J] . Gene therapy . 2020,第3a4期

机译：收缩注意：使用杆状病毒载体遗传修饰的人胚胎干细胞衍生神经干细胞对胶质瘤的目标自杀基因治疗。
2. Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice. [J] . A Stacey, A Schnieke, J McWhir, Molecular and Cellular Biology . 1994,第2期

机译：在胚胎干细胞和小鼠中使用双重置换基因靶向替代人类α-乳清蛋白基因的鼠类。
3. Enhanced transgene expression in primitive hematopoietic progenitor cells and embryonic stem cells efficiently transduced by optimized retroviral hybrid vectors. [J] . Ketteler R, Glaser S, Sandra O, Gene therapy . 2002,第8期

机译：优化的逆转录病毒杂交载体可有效转导原始造血祖细胞和胚胎干细胞中增强的转基因表达。
4. Expression of Renal Genes in Mouse Embryonic Stem Cell Co-cultured with Fetal Renal cells [C] . Li-Xing Zhang, Yang Wang, An Xie, International Forum on Progress on Post-Genome Technologies(5'IFPT); 20070910-11; Suzhou(CN) . 2007

机译：肾基因在胎肾细胞共培养的小鼠胚胎干细胞中的表达
5. Identification of target genes of the transcription factor Zfx in embryonic stem cells. [D] . Galan, Jose M. 2008

机译：鉴定胚胎干细胞中转录因子Zfx的靶基因。
6. High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. [O] . K R Thomas, C Deng, M R Capecchi 1992

机译：通过使用序列替换载体将高保真基因靶向胚胎干细胞。
7. High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. [O] . Thomas, K R, Deng, C, Capecchi, M R 1992

机译：通过使用序列替换载体将高保真基因靶向胚胎干细胞。

High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.

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