...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A DNA methylation site in the male-specific P450 (Cyp 2d-9) promoter and binding of the heteromeric transcription factor GABP.
【24h】

A DNA methylation site in the male-specific P450 (Cyp 2d-9) promoter and binding of the heteromeric transcription factor GABP.

机译：男性特异性P450（Cyp 2d-9）启动子中的DNA甲基化位点和异源转录因子GABP的结合。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Cyp 2d-9 gene encodes the male-specific steroid 16 alpha-hydroxylase in mouse liver and shares a conserved regulatory element (-100TTCCGGGC-93) with another male-specific Slp promoter. As shown with the Slp promoter (N. Yokomori, R. Moore, and M. Negishi, Proc. Natl. Acad. Sci. USA 92:1302-1306, 1995), the male-preferential demethylation also occurs at CpG/-97 in the Cyp 2d-9 promoter. The transcription factor which specifically binds to the demethylated element has been purified. The peptide sequences reveal that the factor consists of GABP alpha and GABP beta 1 with Ets and Notch motifs, respectively. Both DNase I footprinting and gel shift assays indicate that the bacterially expressed glutathione S-transferase-GABP fusion proteins bind to the regulatory element only when CpG/-97 is demethylated. Moreover, Cyp 2d-9 promoter is trans-activated by coexpression of GABP proteins in HepG2 cells. Given the additional results that CpG/-50 of the female-specific steroid 15 alpha-hydroxylase (Cyp 2a-4) promoter is preferentially demethylated in the females, the sex-specific expressions of the P450 genes correlate very well with DNA demethylation. We also conclude that GABP is a methylation-sensitive transcription factor and is a potential transcription activator of the male-specific Cyp 2d-9 promoter.
机译：Cyp 2d-9基因编码小鼠肝脏中的雄性特异性类固醇16α-羟化酶，并与另一个雄性特异性Slp启动子共享一个保守的调控元件（-100TTCCGGGC-93）。如Slp启动子所示（N.Yokomori，R.Moore和M.Negishi，Proc.Natl.Acad.Sci.USA 92：1302-1306，1995），雄性优先去甲基化也发生在CpG / -97处。在Cyp 2d-9启动子中。特异性结合脱甲基元件的转录因子已被纯化。肽序列显示该因子分别由具有Ets和Notch基序的GABP alpha和GABP beta 1组成。 DNase I足迹分析和凝胶迁移分析均表明，仅当CpG / -97脱甲基时，细菌表达的谷胱甘肽S-转移酶-GABP融合蛋白才与调节元件结合。此外，Cyp 2d-9启动子通过在HepG2细胞中共表达GABP蛋白而被反式激活。考虑到雌性类固醇15α-羟化酶（Cyp 2a-4）启动子的CpG / -50在雌性中优先脱甲基的其他结果，P450基因的性别特异性表达与DNA脱甲基非常相关。我们还得出结论，GABP是甲基化敏感的转录因子，并且是雄性特异性Cyp 2d-9启动子的潜在转录激活因子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号