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首页> 外文期刊>Molecular and Cellular Biology >Two-step activation of meiosis by the mat1 locus in Schizosaccharomyces pombe.
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Two-step activation of meiosis by the mat1 locus in Schizosaccharomyces pombe.

机译：粟酒裂殖酵母中mat1基因座可通过两步激活减数分裂。

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The mat1 locus is a key regulator of both conjugation and meiosis in the fission yeast Schizosaccharomyces pombe. Two alternative DNA segments of this locus, mat1-P and mat1-M, specify the haploid cell types (Plus and Minus). Each segment includes two genes: mat1-P includes mat1-Pc and mat1-Pm, while mat1-M includes mat1-Mc and mat1-Mm. The mat1-Pc and mat1-Mc genes are responsible for establishing the pheromone communication system that mediates conjugation between P and M cells, while all four mat1 genes are required for meiosis in diploid P/M cells. Our understanding of the initiation of meiosis is based largely on indirect observations, and a more precise investigation of these events was required to define the interaction between the mat1 genes. Here we resolve this issue using synthetic pheromones and P/M strains with mutations in either mat1-Pc or mat1-Mc. Our results suggest a model in which the mat1 locus plays two roles in controlling meiosis. In the first instance, the mat1-Pc and mat1-Mc functions are required to produce the mating pheromones and receptors that allow the generation of a pheromone signal. This signal is required to induce the expression of mat1-Pm and mat1-Mm. This appears to be the major pheromone-dependent step in controlling meiosis since ectopic expression of these genes allows meiosis in the absence of mat1-Pc and mat1-Mc. The mat1-Pm and mat1-Mm products complete the initiation of meiosis by activating transcription of the mei3 gene.
机译：mat1基因座是裂殖酵母粟酒裂殖酵母中共轭和减数分裂的关键调节因子。该基因座的两个替代DNA片段，mat1-P和mat1-M，指定了单倍体细胞类型（加号和减号）。每个片段包括两个基因：mat1-P包括mat1-Pc和mat1-Pm，而mat1-M包括mat1-Mc和mat1-Mm。 mat1-Pc和mat1-Mc基因负责建立信息素通讯系统，介导P细胞和M细胞之间的结合，而所有四个mat1基因是二倍体P / M细胞减数分裂所必需的。我们对减数分裂的起始了解主要是基于间接观察，因此需要对这些事件进行更精确的研究以定义mat1基因之间的相互作用。在这里，我们使用合成的信息素和在mat1-Pc或mat1-Mc中具有突变的P / M菌株解决了这个问题。我们的结果提出了一个模型，其中mat1基因座在控制减数分裂中扮演两个角色。在第一种情况下，需要mat1-Pc和mat1-Mc功能来产生可产生信息素信号的交配信息素和受体。诱导mat1-Pm和mat1-Mm表达需要此信号。这似乎是控制减数分裂的主要信息素依赖性步骤，因为这些基因的异位表达使得在不存在mat1-Pc和mat1-Mc的情况下能够减数分裂。 mat1-Pm和mat1-Mm产品通过激活mei3基因的转录来完成减数分裂的启动。

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