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首页> 外文期刊>Molecular and Cellular Biology >The osmoregulatory pathway represses mating pathway activity in Saccharomyces cerevisiae: isolation of a FUS3 mutant that is insensitive to the repression mechanism.
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The osmoregulatory pathway represses mating pathway activity in Saccharomyces cerevisiae: isolation of a FUS3 mutant that is insensitive to the repression mechanism.

机译：渗透调节途径抑制啤酒酵母中的交配途径活性：分离对抑制机制不敏感的FUS3突变体。

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Mitogen-activated protein (MAP) kinase cascades are conserved signal transduction pathways that are required for eukaryotic cells to respond to a variety of stimuli. Multiple MAP kinase pathways can function within a single cell type; therefore, mechanisms that insulate one MAP kinase pathway from adventitious activations by parallel pathways may exist. We have studied interactions between the mating pheromone response and the osmoregulatory (high-osmolarity glycerol response [HOG]) pathways in Saccharomyces cerevisiae which utilize the MAP kinases Fus3p and Hog1p, respectively. Inactivating mutations in HOG pathway kinases cause an increase in the phosphotyrosine content of Fus3p, greater expression of pheromone-responsive genes, and increased sensitivity to growth arrest by pheromone. Therefore, the HOG pathway represses mating pathway activity. In a HOG1+ strain, Fus3p phosphotyrosine increases modestly and transiently following an increase in the extracellular osmolarity; however, it increases to a greater extent and for a sustained duration in a hog1-delta strain. Thus, the HOG-mediated repression of mating pathway activity may insulate the mating pathway from activation by osmotic stress. A FUS3 allele whose gene product is resistant to the HOG-mediated repression of its phosphotyrosine content has been isolated. This mutant encodes an amino acid substitution in the highly conserved DPXDEP motif in subdomain XI. Other investigators have shown that the corresponding amino acid is also mutated in a gain-of-function allele of the MAP kinase encoded by the rolled locus in Drosophila melanogaster. These data suggest that the DPXDEP motif plays a role in the negative regulation of MAP kinases.
机译：丝裂原活化蛋白（MAP）激酶级联反应是真核细胞对多种刺激作出反应所需的保守信号转导途径。多种MAP激酶途径可在单个细胞类型内发挥作用。因此，可能存在使一种MAP激酶途径与平行途径的意外激活隔离的机制。我们已经研究了酿酒酵母中的交配信息素反应与渗透调节（高渗透压甘油反应[HOG]）途径之间的相互作用，所述途径分别利用MAP激酶Fus3p和Hog1p。 HOG途径激酶中的失活突变导致Fus3p的磷酸酪氨酸含量增加，信息素反应性基因的更高表达以及对信息素抑制生长的敏感性增加。因此，HOG途径抑制交配途径活性。在HOG1 +菌株中，Fus3p磷酸酪氨酸在细胞外摩尔渗透压浓度增加后适度和短暂地增加。然而，它在hog1-delta菌株中更大程度地增加并持续持续时间。因此，HOG介导的抑制交配途径活性可以使交配途径免受渗透压的激活。已分离出其基因产物对HOG介导的磷酸酪氨酸含量阻抑具有抗性的FUS3等位基因。该突变体在亚结构域XI的高度保守的DPXDEP基序中编码氨基酸取代。其他研究者已经表明，相应的氨基酸也在果蝇中由滚动基因座编码的MAP激酶的功能获得等位基因中突变。这些数据表明，DPXDEP基序在MAP激酶的负调控中起作用。

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