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首页> 外文期刊>Molecular and Cellular Biology >Different tissue-specific expression of the amylase gene Amy-1 in mice and rats.
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Different tissue-specific expression of the amylase gene Amy-1 in mice and rats.

机译：淀粉酶基因Amy-1在小鼠和大鼠中的不同组织特异性表达。

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We cloned the rat alpha-amylase gene Amy-1 and compared its structure and expression with its mouse counterpart. The results showed that the general organization of the transcriptionally active rat Amy-1 gene was similar to that of its mouse counterpart; i.e., the rat gene also contained two independent transcriptional promoters. The distance between the two promoters in the rat gene was, however, more than double (6 kilobases) that measured in the mouse gene (2.8 kilobases). In addition, the rat genome also contained an independent, orphonlike version of the weaker Amy-1 promoter, which was transcriptionally silent. In spite of the similar overall organization of the Amy-1 genes in mouse and rat cells, an interesting difference was observed in the expression of the weak promoter in these two closely related rodents. In rats this promoter was significantly active only in liver cells, while in mice it was utilized with similar efficiencies in parotid, liver, and pancrease cells. Moreover, the transcripts produced in rat liver had a very heterogeneous population of 5' ends, located between 180 and 220 nucleotides upstream of the two homologous start sites observed for this promoter in mouse liver, even though the sequences around this region were strongly conserved between the two species.
机译：我们克隆了大鼠α-淀粉酶基因Amy-1，并将其结构和表达与其小鼠对应物进行了比较。结果表明，具有转录活性的大鼠Amy-1基因的总体结构与其小鼠相似。即，大鼠基因还包含两个独立的转录启动子。然而，大鼠基因中两个启动子之间的距离是小鼠基因中（2.8 KB）的两倍（6 KB）。此外，大鼠基因组还包含一个较弱的Amy-1启动子的独立的，类似Orphonlike的形式，后者在转录上是沉默的。尽管在小鼠和大鼠细胞中Amy-1基因的总体结构相似，但在这两个密切相关的啮齿动物中，弱启动子的表达却观察到了有趣的差异。在大鼠中，该启动子仅在肝细胞中具有显着活性，而在小鼠中，腮腺，肝和胰脏细胞中的启动子具有相似的效率。此外，在大鼠肝脏中产生的转录本具有非常不同的5'末端群体，位于在小鼠肝脏中观察到的该启动子的两个同源起始位点的上游180和220个核苷酸之间，即使该区域周围的序列之间是高度保守的。这两种。

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