Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

F Toneguzzo; A C Hayday; A Keating

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Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

机译：电场介导的DNA转移：人和小鼠淋巴样细胞中瞬时稳定的基因表达。

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The technique of DNA transfer by electroporation was investigated in an effort to evaluate its utility for the identification of developmentally controlled regulatory sequences. Transient and stable gene expression was detected in a variety of lymphoid cell lines subjected to electroporation. No correlation existed between the levels of chloramphenicol acetyltransferase (acetyl-CoA; chloramphenicol 3-O-acetyltransferase, EC 2.3.1.28) expression and stable transfection frequency. In all lymphoid cell lines tested, the simian virus 40 early region was a better promoter than was the Rous sarcoma virus long terminal repeat.

机译：为了评估其用于鉴定发育受控的调控序列的效用，研究了通过电穿孔进行DNA转移的技术。在经过电穿孔的各种淋巴样细胞系中检测到瞬时稳定的基因表达。氯霉素乙酰转移酶（乙酰辅酶A;氯霉素3-O-乙酰转移酶，EC 2.3.1.28）的表达水平与稳定的转染频率之间没有相关性。在所有测试的淋巴样细胞系中，猿猴病毒40早期区域是比劳斯肉瘤病毒长末端重复序列更好的启动子。

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《Molecular and Cellular Biology》 |1986年第2期|共4页
作者
F Toneguzzo; A C Hayday; A Keating;
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中图分类细胞生物学;
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1. Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells [J] . A. Keating, F. Toneguzzo, K. McDonald, Nucleic acids research . 1988,第12期

机译：电场介导的基因转移：淋巴细胞转移中DNA转移和整合模式的表征
2. Overexpression of stably transfected human glutathione S-transferase P1-1 protects against DNA damage by benzo(a)pyrene diol-epoxide in human T47D cells. [J] . Fields WR, Morrow CS, Doss AJ, Molecular pharmacology. . 1998,第2期

机译：稳定转染的人谷胱甘肽S-转移酶P1-1的过度表达可防止人T47D细胞中苯并（a）re二醇-环氧化合物对DNA的损害。
3. Satellite DNA induces unstable expression of the adjacent herpes simplex virus tk gene cotransfected in mouse cells. [J] . D Talarico, A F Peverali, E Ginelli, Molecular and Cellular Biology . 1988,第3期

机译：卫星DNA诱导在小鼠细胞中共转染的相邻单纯疱疹病毒tk基因的不稳定表达。
4. The effects benzo(a)pyrenediolexpoxide exposure on DNA adduct formation, altered gene expression, and toxicity in human lymphoblastoid TK6 cells. [C] . Joshua J Klaene, Jennifer Barrett, Caroline Ceailles Flakaros, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：苯并（a）Pyrenyiolexpatiene暴露在DNA加合物形成，改变基因表达和人淋巴细胞Tk6细胞中的效果暴露。
5. Transient and stable expression of human beta-galactosidase and protective protein in COS-1 and CHO cells. [D] . Yaghi, Farhan F. 1993

机译：人β-半乳糖苷酶和保护蛋白在COS-1和CHO细胞中的瞬时稳定表达。
6. Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells. [O] . F Toneguzzo, A C Hayday, A Keating 1986

机译：电场介导的DNA转移：人和小鼠淋巴样细胞中瞬时稳定的基因表达。
7. Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells. [O] . Toneguzzo, F, Hayday, A C, Keating, A 1986

机译：电场介导的DNA转移：人和小鼠淋巴样细胞中瞬时稳定的基因表达。

Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells.

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