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首页> 外文期刊>Molecular and Cellular Biology >mRNA Capping Enzyme Activity Is Coupled to an Early Transcription Elongation
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mRNA Capping Enzyme Activity Is Coupled to an Early Transcription Elongation

机译：mRNA上限酶活性与早期转录伸长率相关。

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One of the temperature-sensitive alleles of CEG1, a guanylyltransferase subunit of the Saccharomyces cerevisiae capping enzyme, showed 6-azauracil (6AU) sensitivity at the permissive growth temperature, which is a phenotype that is correlated with a transcription elongation defect. This temperature-sensitive allele, ceg1-63, has an impaired ability to induce PUR5 in response to 6AU treatment and diminished enzyme-GMP formation activity. However, this cellular and molecular defect is not primarily due to the preferential degradation of the transcript attributed to a lack of cap structure. Our data suggest that the guanylyltransferase subunit of the capping enzyme plays a role in transcription elongation as well as cap formation. First, in addition to the 6AU sensitivity, ceg1-63 is synthetically lethal with elongation-defective mutations in RNA polymerase II. Secondly, it produces a prolonged steady-state level of GAL1 mRNA after glucose shutoff. Third, it decreases the transcription read through a tandem array of promoter-proximal pause sites in an orientation-dependent manner. Taken together, we present direct evidence that suggests a role of capping enzyme in an early transcription. Capping enzyme ensures the early transcription checkpoint by capping of the nascent transcript in time and allowing it to extend further.
机译： CEG1 的温度敏感性等位基因之一是酿酒酵母（Saccharomyces cerevisiae）封帽酶的鸟苷基转移酶亚基，在允许的生长温度下显示6-氮杂嘧啶（6AU）敏感性，即与转录延伸缺陷相关的表型。温度敏感的等位基因 ceg1 - 63 响应6AU处理而诱导 PUR5 的能力受损，并且酶GMP形成活性降低。然而，这种细胞和分子缺陷不是主要由于缺乏帽结构而导致的转录物优先降解。我们的数据表明，封端酶的鸟苷基转移酶亚基在转录延伸和帽形成中起作用。首先，除了6AU敏感性外， ceg1 - 63 在RNA聚合酶II中具有延伸缺陷型突变，具有合成致命性。其次，它在葡萄糖切断后产生延长的 GAL1 mRNA稳态水平。第三，它以依赖于方向的方式减少了通过串联的启动子近端暂停位点阵列读取的转录。综上所述，我们提供了直接的证据，表明加帽酶在早期转录中的作用。封端酶通过及时封盖新生转录本并使其进一步延伸来确保早期转录检查点。

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