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首页> 外文期刊>Molecular and Cellular Biology >MyoD and myogenin act on the chicken myosin light-chain 1 gene as distinct transcriptional factors.
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MyoD and myogenin act on the chicken myosin light-chain 1 gene as distinct transcriptional factors.

机译：MyoD和肌生成素作为独特的转录因子作用于鸡肌球蛋白轻链1基因。

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Expression of MyoD, myogenin, MRF4, and Myf-5 converts nonmuscle cells to muscle cells. In an attempt to analyze the roles of these factors, we have investigated their effects on transcription driven by the promoter of the chicken myosin alkaline light-chain (MLC1) gene. The activation by CMD1 or c-myogenin (chicken MyoD or myogenin, respectively) was dependent on the existence of a muscle-specific regulatory region located from positions -2096 to -1743. Its distal half, containing a pair of E boxes (CANNTG), had been previously characterized as an enhancer responsive to CMD1 but not to c-myogenin. In this study, we report the identification of another enhancer in the muscle-specific regulatory region which is preferentially responsive to c-myogenin. Deletion and mutation analyses indicated that this enhancer requires a single E box and its flanking sequences. Furthermore, analysis of chimeric proteins of CMD1 and c-myogenin indicated that regions outside the basic helix-loop-helix domain of c-myogenin are involved in the specificity of the enhancer. These results show that CMD1 and c-myogenin act on the MLC1 gene by recognizing different upstream DNA sequences and that direct or indirect interactions between the regions outside the basic helix-loop-helix domain and flanking sequences of E boxes are involved in the target sequence specificity.
机译：MyoD，肌生成素，MRF4和Myf-5的表达将非肌肉细胞转化为肌肉细胞。为了分析这些因素的作用，我们研究了它们对鸡肌球蛋白碱性轻链（MLC1）基因启动子驱动的转录的影响。 CMD1或c-肌生成素（分别为鸡MyoD或肌生成素）的激活取决于位于-2096至-1743位的肌肉特异性调节区的存在。它的远端一半包含一对E盒（CANNTG），先前已被描述为对CMD1敏感但对c-肌生成素无反应的增强子。在这项研究中，我们报告了在肌肉特异性调节区域中优先对c-肌生成素作出反应的另一种增强剂的鉴定。缺失和突变分析表明该增强子需要单个E框及其侧翼序列。此外，对CMD1和c-肌生成素的嵌合蛋白的分析表明，c-肌生成素的基本螺旋-环-螺旋结构域之外的区域与增强子的特异性有关。这些结果表明，CMD1和c-肌生成素通过识别不同的上游DNA序列而作用于MLC1基因，并且靶序列中涉及基本螺旋-环-螺旋结构域外部区域与E盒侧翼序列之间的直接或间接相互作用。特异性。

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