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首页> 外文期刊>Molecular and Cellular Biology >The ribosomal L5 protein is associated with mdm-2 and mdm-2-p53 complexes.
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The ribosomal L5 protein is associated with mdm-2 and mdm-2-p53 complexes.

机译：核糖体L5蛋白与mdm-2和mdm-2-p53复合物相关。

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Throughout the purification of the mdm-2 or mdm-2-p53 protein complexes, a protein with a molecular weight of 34,000 was observed to copurify with these proteins. Several monoclonal antibodies directed against distinct epitopes in the mdm-2 or p53 protein coimmunoprecipitated this 34,000-molecular-weight protein, which did not react to p53 or mdm-2 polyclonal antisera in a Western immunoblot. The N-terminal amino acid sequence of this 34,000-molecular-weight protein demonstrated that the first 40 amino acids were identical to the ribosomal L5 protein, found in the large rRNA subunit and bound to 5S RNA. Partial peptide maps of the authentic L5 protein and the 34,000-molecular-weight protein were identical. mdm-2-L5 and mdm-2-L5-p53 complexes were shown to bind 5S RNA specifically, presumably through the known specificity of L5 protein for 5S RNA. In 5S RNA-L5-mdm-2-p53 ribonucleoprotein complexes, it was also possible to detect the 5.8S RNA which has been suggested to be covalently linked to a percentage of the p53 protein in a cell. These experiments have identified a unique ribonucleoprotein complex composed of 5S RNA, L5 protein, mdm-2 proteins, p53 protein, and possibly the 5.8S RNA. While the function of such a ribonucleoprotein complex is not yet clear, the identity of its component parts suggests a role for these proteins and RNA species in ribosomal biogenesis, ribosomal transport from the nucleus to the cytoplasm, or translational regulation in the cell.
机译：在整个mdm-2或mdm-2-p53蛋白复合物的纯化过程中，观察到分子量为34,000的蛋白与这些蛋白共纯化。几种针对mdm-2或p53蛋白中不同表位的单克隆抗体共同免疫沉淀了这种34,000分子量的蛋白，在Western免疫印迹中，该蛋白不与p53或mdm-2多克隆抗血清反应。该34,000分子量蛋白的N末端氨基酸序列证明前40个氨基酸与核糖体L5蛋白相同，存在于较大的rRNA亚基中并与5S RNA结合。真实的L5蛋白和34,000分子量蛋白的部分肽图是相同的。显示mdm-2-L5和mdm-2-L5-p53复合物特异性结合5S RNA，大概是通过L5蛋白对5S RNA的已知特异性。在5S RNA-L5-mdm-2-p53核糖核蛋白复合物中，还可以检测到5.8S RNA，该5.8S RNA被认为与细胞中一定比例的p53蛋白共价连接。这些实验已经鉴定出由5S RNA，L5蛋白，mdm-2蛋白，p53蛋白以及可能的5.8S RNA组成的独特核糖核蛋白复合物。虽然这种核糖核蛋白复合物的功能尚不清楚，但其组成部分的同一性暗示了这些蛋白质和RNA在核糖体生物发生，核糖体从核到细胞质的转运或细胞中的翻译调节中的作用。

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