...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Evidence that Negative Elongation Factor Represses Transcription Elongation through Binding to a DRB Sensitivity-Inducing Factor/RNA Polymerase II Complex and RNA
【24h】

Evidence that Negative Elongation Factor Represses Transcription Elongation through Binding to a DRB Sensitivity-Inducing Factor/RNA Polymerase II Complex and RNA

机译：负伸长因子通过结合DRB敏感性诱导因子/ RNA聚合酶II复合物和RNA抑制转录伸长的证据。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Negative elongation factor (NELF) is a human transcription factor complex that cooperates with DRB sensitivity-inducing factor (DSIF)/hSpt4-hSpt5 to repress elongation by RNA polymerase II (RNAPII). NELF activity is associated with five polypeptides, including NELF-A, a candidate gene product for Wolf-Hirschhorn syndrome, and NELF-E, a putative RNA-binding protein with arginine-aspartic acid (RD) dipeptide repeats. Here we report several important findings regarding the DSIF/NELF-dependent elongation control. First, we have established an effective method for purifying the active NELF complex using an epitope-tagging technique. Second, the five polypeptides each are important and together are sufficient for its function in vitro. Third, NELF does not bind to either DSIF or RNAPII alone but does bind to the preformed DSIF/RNAPII complex. Fourth, NELF-E has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions. Taken together, we propose that NELF causes RNAPII pausing through binding to the DSIF/RNAPII complex and to nascent transcripts. These results also have implications for how DSIF and NELF are regulated in a gene-specific manner in vivo.
机译：负伸长因子（NELF）是一种人类转录因子复合物，可与DRB敏感性诱导因子（DSIF）/ hSpt4-hSpt5协同抑制RNA聚合酶II（RNAPII）的伸长。 NELF活性与五种多肽有关，包括NELF-A（沃尔夫-赫希霍恩综合征的候选基因产物）和NELF-E（一种假定的RNA结合蛋白，带有精氨酸-天冬氨酸（RD）二肽重复序列）。在这里，我们报告了有关DSIF / NELF依赖的伸长率控制的几个重要发现。首先，我们已经建立了使用表位标记技术纯化活性NELF复合物的有效方法。第二，这五种多肽都很重要，并且在一起足以在体外发挥作用。第三，NELF不能单独与DSIF或RNAPII结合，但可以与预先形成的DSIF / RNAPII复合物结合。第四，NELF-E具有功能性的RNA结合结构域，其突变会削弱转录抑制，而不影响已知的蛋白质-蛋白质相互作用。综上所述，我们认为NELF通过与DSIF / RNAPII复合体和新生转录本结合而引起RNAPII暂停。这些结果对于体内如何以基因特异性方式调节DSIF和NELF也具有意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号