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首页> 外文期刊>Molecular and Cellular Biology >Yeast Protein Kinases and the RHO1 Exchange Factor TUS1 Are Novel Components of the Cell Integrity Pathway in Yeast
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Yeast Protein Kinases and the RHO1 Exchange Factor TUS1 Are Novel Components of the Cell Integrity Pathway in Yeast

机译：酵母蛋白激酶和RHO1交换因子TUS1是酵母中细胞完整性途径的新组成部分。

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摘要

The PKC1-associated mitogen-activated protein (MAP) kinase pathway of Saccharomyces cerevisiae regulates cell integrity by controlling the actin cytoskeleton and cell wall synthesis. Activation of PKC1 occurs via the GTPase RHO1 and the kinase pair PKH1 and PKH2. Here we report that YPK1 and YPK2, an essential pair of homologous kinases and proposed downstream effectors of PKH and sphingolipids, are also regulators of the PKC1-controlled MAP kinase cascade. ypk mutants display random distribution of the actin cytoskeleton and severely reduced activation of the MAP kinase MPK1. Upregulation of the RHO1 GTPase switch or the PKC1 effector MAP kinase pathway suppresses the growth and actin defects of ypk cells. ypk lethality is also suppressed by overexpression of an uncharacterized gene termed TUS1. TUS1 is a novel RHO1 exchange factor that contributes to cell wall integrity-mediated modulation of RHO1 activity. Thus, TUS1 and the YPKs add to the growing complexity of RHO1 and PKC1 regulation in the cell integrity signaling pathway. Furthermore, our findings suggest that the YPKs are a missing link between sphingolipid signaling and the cell integrity pathway.
机译：酿酒酵母的PKC1相关的丝裂原活化蛋白（MAP）激酶途径通过控制肌动蛋白的细胞骨架和细胞壁合成来调节细胞完整性。 PKC1的激活通过GTPase RHO1和激酶对PKH1和PKH2发生。在这里我们报告YPK1和YPK2，必不可少的一对同源激酶和PKH和鞘脂的下游效应器，也是PKC1控制的MAP激酶级联的调节剂。 ypk 突变体显示肌动蛋白细胞骨架的随机分布，并严重降低了MAP激酶MPK1的激活。 RHO1 GTPase开关或PKC1效应子MAP激酶途径的上调抑制了 ypk 细胞的生长和肌动蛋白缺陷。 ypk 的致死性也被称为 TUS1 的未表征基因的过表达抑制。 TUS1是一种新型的RHO1交换因子，有助于细胞壁完整性介导的RHO1活性的调节。因此，TUS1和YPKs在细胞完整性信号通路中增加了RHO1和PKC1调控的复杂性。此外，我们的发现表明YPKs是鞘脂信号传导与细胞完整性途径之间的缺失环节。

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