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Sbp1p Affects Translational Repression and Decapping in Saccharomyces cerevisiae

机译：Sbp1p影响啤酒酵母的翻译抑制和去盖化。

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The relationship between translation and mRNA turnover is critical to the regulation of gene expression. One major pathway for mRNA turnover occurs by deadenylation, which leads to decapping and subsequent 5′-to-3′ degradation of the body of the mRNA. Prior to mRNA decapping, a transcript exits translation and enters P bodies to become a potential decapping substrate. To understand the transition from translation to decapping, it is important to identify the factors involved in this process. In this work, we identify Sbp1p (formerly known as Ssb1p), an abundant RNA binding protein, as a high-copy-number suppressor of a conditional allele in the decapping enzyme. Sbp1p overexpression restores normal decay rates in decapping-defective strains and increases P-body size and number. In addition, Sbp1p promotes translational repression of mRNA during glucose deprivation. Moreover, P-body formation is reduced in strains lacking Sbp1p. Sbp1p acts in conjunction with Dhh1p, as it is required for translational repression and P-body formation in pat1Δ strains under these conditions. These results identify Sbp1p as a new protein that functions in the transition of mRNAs from translation to an mRNP complex destined for decapping.
机译：翻译和mRNA更新之间的关系对于调节基因表达至关重要。 mRNA转化的一种主要途径是通过去甲腺苷酸化作用，这导致脱盖以及随后mRNA体内的5'至3'降解。在mRNA脱盖之前，转录本会退出翻译并进入P体，成为潜在的脱盖底物。要了解从翻译到封顶的过渡，确定此过程中涉及的因素很重要。在这项工作中，我们确定了Sbp1p（以前称为Ssb1p），一种丰富的RNA结合蛋白，可以作为脱保护酶中条件等位基因的高拷贝数抑制剂。 Sbp1p的过表达恢复了去盖缺陷菌株的正常衰变速率，并增加了P体的大小和数量。此外，Sbp1p在葡萄糖剥夺过程中促进mRNA的翻译抑制。此外，缺乏Sbp1p的菌株P体形成减少。 Sbp1p与Dhh1p共同起作用，这是在这些条件下 pat1 Δ菌株中翻译抑制和P体形成所必需的。这些结果确定了Sbp1p是一种新蛋白，可在mRNA从翻译到mRNP复合体的过渡中发挥作用，该复合体将用于去盖。

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