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首页> 外文期刊>Molecular and Cellular Biology >Spn1 Regulates the Recruitment of Spt6 and the Swi/Snf Complex during Transcriptional Activation by RNA Polymerase II
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Spn1 Regulates the Recruitment of Spt6 and the Swi/Snf Complex during Transcriptional Activation by RNA Polymerase II

机译：Spn1通过RNA聚合酶II调节转录激活过程中Spt6和Swi / Snf复合物的招聘。

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We investigated the timing of the recruitment of Spn1 and its partner, Spt6, to the CYC1 gene. Like TATA binding protein and RNA polymerase II (RNAPII), Spn1 is constitutively recruited to the CYC1 promoter, although levels of transcription from this gene, which is regulated postrecruitment of RNAPII, are low. In contrast, Spt6 appears only after growth in conditions in which the gene is highly transcribed. Spn1 recruitment is via interaction with RNAPII, since an spn1 mutant defective for interaction with RNAPII is not targeted to the promoter, and Spn1 is necessary for Spt6 recruitment. Through a targeted genetic screen, strong and specific antagonizing interactions between SPN1 and genes encoding Swi/Snf subunits were identified. Like Spt6, Swi/Snf appears at CYC1 only after activation of the gene. However, Spt6 significantly precedes Swi/Snf occupancy at the promoter. In the absence of Spn1 recruitment, Swi/Snf is constitutively found at the promoter. These observations support a model whereby Spn1 negatively regulates RNAPII transcriptional activity by inhibiting recruitment of Swi/Snf to the CYC1 promoter, and this inhibition is abrogated by the Spn1-Spt6 interaction. These findings link Spn1 functions to the transition from an inactive to an actively transcribing RNAPII complex at a postrecruitment-regulated promoter.
机译：我们研究了将Spn1及其伴侣Spt6募集到 CYC1 基因的时间。与TATA结合蛋白和RNA聚合酶II（RNAPII）一样，Spn1组成型地募集到 CYC1 启动子，尽管该基因的转录水平受招聘后的RNAPII调控。相反，Spt6仅在基因高度转录的条件下生长后出现。 Spn1募集是通过与RNAPII的相互作用进行的，因为与RNAPII相互作用存在缺陷的 spn1 突变体并不针对启动子，而Spn1对于Spt6的募集是必需的。通过有针对性的遗传筛选，确定了 SPN1 与编码Swi / Snf亚基的基因之间的强烈和特异性拮抗相互作用。与Spt6一样，Swi / Snf仅在基因激活后才出现在 CYC1 上。但是，Spt6在启动子之前先于Swi / Snf占据。在没有Spn1募集的情况下，在启动子上组成性地发现Swi / Snf。这些观察结果支持Spn1通过抑制Swi / Snf募集到 CYC1 启动子来负调控RNAPII转录活性的模型，而Spn1-Spt6相互作用则消除了这种抑制作用。这些发现将Spn1功能与在招聘后调节的启动子处从无活性的RNAPII复合物过渡到活性的转录有关。

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