...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Components of the SAGA Histone Acetyltransferase Complex Are Required for Repressed Transcription of ARG1 in Rich Medium
【24h】

Components of the SAGA Histone Acetyltransferase Complex Are Required for Repressed Transcription of ARG1 in Rich Medium

机译：SAGA组蛋白乙酰基转移酶复合物的成分对于富含培养基中ARG1的转录抑制是必需的

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transcriptional regulation of the Saccharomyces cerevisiae ARG1 gene is controlled by positive and negative elements. The transactivator Gcn4p is required for activation in minimal medium, while arginine repression requires the ArgR/Mcm1 regulatory complex, which binds to two upstream arginine control elements. We have found that the coordinated regulation of ARG1 requires components of the SAGA chromatin-remodeling complex. Using gcn5 deletion strains and a Gcn5 protein carrying the E173Q mutation in the histone acetyltransferase (HAT) region, we show that the HAT activity of Gcn5p is required for repression of ARG1 in rich medium. Similar increases in expression were seen upon deletion of other SAGA components but not upon deletion of the ADA-specific component, Ahc1p. Chromatin immunoprecipitations using antibodies to acetylated H3 confirmed that a decrease in the level of acetylated histones at the ARG1 promoter correlated with increased ARG1 expression. Up-regulation of ARG1 in the absence of Gcn5p also correlated with increased binding of TATA-binding protein to the promoter. The analysis of promoter deletions showed that Gcn5/Ada repression of ARG1 was mediated through the action of the ArgR/Mcm1 regulatory complex. In addition, studies with minimal medium demonstrated a requirement for the Ada proteins in activation of ARG1. This suggests that SAGA has a dual role at ARG1, acting to repress transcription in rich medium and activate transcription in minimal medium.
机译：酿酒酵母ARG1 基因的转录调控受阳性和阴性因素的控制。反式激活剂Gcn4p是在最小培养基中激活所必需的，而精氨酸抑制则需要ArgR / Mcm1调节复合物，该复合物与两个上游精氨酸控制元件结合。我们发现， ARG1 的协调调控需要SAGA染色质重塑复合物的组成部分。使用 gcn5 缺失菌株和在组蛋白乙酰转移酶（HAT）区域携带E173Q突变的Gcn5蛋白，我们证明Gcn5p的HAT活性对于抑制 ARG1 丰富的媒体。删除其他SAGA组件后，会看到类似的表达增加，但删除ADA特定组件Ahc1p时却没有。使用针对乙酰化H3的抗体进行的染色质免疫沉淀证实，在 ARG1 启动子上乙酰化组蛋白水平的降低与 ARG1 表达的增加有关。在缺少Gcn5p的情况下， ARG1 的上调也与TATA结合蛋白与启动子的结合增加有关。启动子缺失的分析表明， ARG1 的Gcn5 / Ada抑制是通过ArgR / Mcm1调控复合物的作用介导的。此外，用最少的培养基进行的研究表明， ARG1 的激活需要Ada蛋白。这表明SAGA在 ARG1 中具有双重作用，可抑制丰富培养基中的转录并激活基本培养基中的转录。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号