The GLI gene encodes a nuclear protein which binds specific sequences in the human genome.

K W Kinzler; B Vogelstein

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The GLI gene encodes a nuclear protein which binds specific sequences in the human genome.

机译：GLI基因编码一种核蛋白，该蛋白与人类基因组中的特定序列结合。

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The GLI gene is amplified in a subset of human tumors and encodes a protein product with five zinc finger DNA-binding motifs. In this study, we show that the GLI gene product has a predominantly nuclear localization and binds DNA in a sequence-specific fashion. Three GLI binding sites were identified by using a novel procedure in which total human DNA was bound to a GLI recombinant fusion protein, and the polymerase chain reaction was used to amplify and recover the bound sequences. The GLI protein protected a 23- to 24-base region within all three binding sites, and the protected region in each case included the 9-base-pair sequence 5'-GACCACCCA-3'. One of the binding sites was contained within a 63-base-pair repeat of the variable number of tandem repeat type, whereas the other two sites were represented once in the genome. The approach used here to identify GLI binding sites should be applicable to the characterization of other zinc finger proteins.

机译：GLI基因在人类肿瘤的一部分中被扩增，并编码具有五个锌指DNA结合基序的蛋白质产物。在这项研究中，我们显示GLI基因产物主要具有核定位，并以序列特异性方式结合DNA。通过使用一种新颖的方法鉴定了三个GLI结合位点，其中总的人类DNA与GLI重组融合蛋白结合，并且聚合酶链反应用于扩增和回收结合的序列。 GLI蛋白保护所有三个结合位点内的23到24个碱基区域，并且每种情况下的受保护区域都包含9个碱基对的5'-GACCACCCA-3'序列。结合位点之一包含在可变数目的串联重复序列类型的63个碱基对的重复序列内，而其他两个位点在基因组中仅代表一次。此处用于鉴定GLI结合位点的方法应适用于其他锌指蛋白的表征。

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《Molecular and Cellular Biology》 |1990年第2期|共9页
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K W Kinzler; B Vogelstein;
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中图分类细胞生物学;
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6. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. [O] . K W Kinzler, B Vogelstein 1990

机译：GLI基因编码与人类基因组中特定序列结合的核蛋白。
7. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome. [O] . Kinzler K W, Vogelstein B 1990

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The GLI gene encodes a nuclear protein which binds specific sequences in the human genome.

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