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首页> 外文期刊>Molecular and Cellular Biology >Identification of putative c-Myc-responsive genes: characterization of rcl, a novel growth-related gene.
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Identification of putative c-Myc-responsive genes: characterization of rcl, a novel growth-related gene.

机译：推定的c-Myc反应基因的鉴定：rcl的表征，一种新的与生长相关的基因。

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The c-Myc protein is a helix-loop-helix leucine zipper oncogenic transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. The biochemical function of c-Myc has been well described, yet the identities of downstream effectors are just beginning to emerge. We describe the identification of a set of c-Myc-responsive genes in the Rat1a fibroblast through the application of cDNA representational difference analysis (RDA) to cDNAs isolated from nonadherent Rat1a and Rat1a-myc cells. In this system, c-Myc overexpression is sufficient to induce the transformed phenotype of anchorage-independent growth. We identified 20 differentially expressed cDNAs, several of which represent novel cDNA sequences. We further characterized one of the novel cDNAs identified in this screen, termed rcl. rcl expression is (i) directly stimulated by c-Myc; (ii) stimulated in the in vivo growth system of regenerating rat liver, as is c-myc; and (iii) elevated in human lymphoid cells that overexpress c-myc. By using an anti-Rcl antibody, immunoblot analysis, and immunofluorescence microscopy, the Rcl protein was found to be a 23-kDa nuclear protein. Ectopic expression of the protein encoded by the rcl cDNA induces anchorage-independent growth in Rat1a fibroblasts, albeit to a diminished extent compared to ectopic c-Myc expression. These data suggest a role for rcl during cellular proliferation and c-Myc-mediated transformation.
机译：c-Myc蛋白是螺旋-环-螺旋亮氨酸拉链致癌转录因子，参与细胞增殖，分化和凋亡的调控。 c-Myc的生化功能已被很好地描述，但下游效应子的身份才刚刚开始出现。我们描述了通过对非粘附的Rat1a和Rat1a-myc细胞分离的cDNA应用cDNA代表性差异分析（RDA），在Rat1a成纤维细胞中鉴定一组c-Myc反应基因。在此系统中，c-Myc过表达足以诱导依赖于锚定的生长的转化表型。我们鉴定了20个差异表达的cDNA，其中几个代表新的cDNA序列。我们进一步表征了在此筛选中鉴定的一种新型cDNA，称为rcl。 rcl表达（i）由c-Myc直接刺激; （ii）刺激大鼠肝脏再生的体内生长系统，如c-myc一样; （iii）过度表达c-myc的人类淋巴样细胞升高。通过使用抗Rcl抗体，免疫印迹分析和免疫荧光显微镜检查，发现Rcl蛋白是23 kDa核蛋白。由rcl cDNA编码的蛋白质的异位表达在Rat1a成纤维细胞中诱导锚定非依赖性生长，尽管与异位c-Myc表达相比程度有所降低。这些数据表明rcl在细胞增殖和c-Myc介导的转化过程中的作用。

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