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首页> 外文期刊>Molecular and Cellular Biology >Yeast Rmi1/Nce4 Controls Genome Stability as a Subunit of the Sgs1-Top3 Complex
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Yeast Rmi1/Nce4 Controls Genome Stability as a Subunit of the Sgs1-Top3 Complex

机译：酵母Rmi1 / Nce4作为Sgs1-Top3复合体的一个亚基控制基因组稳定性。

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Genome stability requires a set of RecQ-Top3 DNA helicase-topoisomerase complexes whose sole budding yeast homolog is encoded by SGS1-TOP3. RMI1/NCE4 was identified as a potential intermediate in the SGS1-TOP3 pathway, based on the observation that strains lacking any one of these genes require MUS81 and MMS4 for viability. This idea was tested by confirming that sgs1 and rmi1 mutants display the same spectrum of synthetic lethal interactions, including the requirements for SLX1, SLX4, SLX5, and SLX8, and by demonstrating that rmi1 mus81 synthetic lethality is dependent on homologous recombination. On their own, mutations in RMI1 result in phenotypes that mimic those of sgs1 or top3 strains including slow growth, hyperrecombination, DNA damage sensitivity, and reduced sporulation. And like top3 strains, most rmi1 phenotypes are suppressed by mutations in SGS1. We show that Rmi1 forms a heteromeric complex with Sgs1-Top3 in yeast and that these proteins interact directly in a recombinant system. The Rmi1-Top3 complex is stable in the absence of the Sgs1 helicase, but the loss of either Rmi1 or Top3 in yeast compromises its partner's interaction with Sgs1. Biochemical studies demonstrate that recombinant Rmi1 is a structure-specific DNA binding protein with a preference for cruciform structures. We propose that the DNA binding specificity of Rmi1 plays a role in targeting Sgs1-Top3 to appropriate substrates.
机译：基因组稳定性需要一套RecQ-Top3 DNA解旋酶-拓扑异构酶复合物，其唯一的出芽酵母同源物由 SGS1-TOP3编码。 RMI1 / NCE4 被认为是 SGS1-TOP3 途径中的潜在中间产物，这是由于观察到缺乏这些基因之一的菌株需要 MUS81 和< em> MMS4 。通过确认 sgs1 和 rmi1 突变体显示出相同的合成致死作用谱，包括对 SLX1 ，的要求，对这一想法进行了测试。 SLX4 ， SLX5 和 SLX8 ，并证明 rmi1 mus81 的合成杀伤力取决于同源重组。就其本身而言， RMI1 的突变会导致表型模仿 sgs1 或 top3 菌株，包括生长缓慢，超重组，DNA损伤敏感性和减少孢子形成。与 top3 菌株一样，大多数 rmi1 表型也受到 SGS1 突变的抑制。我们显示Rmi1在酵母中与Sgs1-Top3形成异源复合物，并且这些蛋白质在重组系统中直接相互作用。 Rmi1-Top3复合物在没有Sgs1解旋酶的情况下是稳定的，但是酵母中Rmi1或Top3的丢失会损害其伴侣与Sgs1的相互作用。生化研究表明，重组Rmi1是一种结构特异性的DNA结合蛋白，对十字形结构具有偏爱。我们建议Rmi1的DNA结合特异性在将Sgs1-Top3靶向适当的底物上发挥作用。

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