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Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae

机译：酿酒酵母核内正常mRNA的降解

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A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Δ strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-Δ strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-Δ, rai1-Δ, and cbc1-Δ deletions, but not by the upf1-Δ deletion, suggesting that DRN requires Rrp6p, a 3′-to-5′ nuclear exonuclease, the Rat1p, a 5′-to-3′ nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3′ end formation defects in certain mutants.
机译：通过分析 Saccharomyces cerevisiae 的nup116-Δ菌株缺乏输出所有RNA（包括poly（A））的能力，确定了核mRNA降解（DRN）系统。 ）在限制温度下的mRNA。在nup116-Δ菌株中进行Northern印迹，原位杂交并用硫菌素阻止转录，揭示了 rrp6 -Δ，< em> rai1 -Δ和 cbc1 -Δ缺失，但不是 upf1-Δ缺失，这表明DRN需要Rrp6p（3'- -5'核外切核酸酶Rat1p是5'-to-3'核外切核酸酶，Cbc1p是CBC的核帽结合复合物，可将mRNA引导至降解位点。我们建议保留在细胞核中的某些正常mRNA被DRN系统降解，类似于某些突变体中具有3'末端形成缺陷的转录物的降解。

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