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首页> 外文期刊>Molecular and Cellular Biology >Mutations in a Partitioning Protein and Altered Chromatin Structure at the Partitioning Locus Prevent Cohesin Recruitment by the Saccharomyces cerevisiae Plasmid and Cause Plasmid Missegregation
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Mutations in a Partitioning Protein and Altered Chromatin Structure at the Partitioning Locus Prevent Cohesin Recruitment by the Saccharomyces cerevisiae Plasmid and Cause Plasmid Missegregation

机译：分区蛋白上的突变蛋白和染色质结构的突变会阻止酿酒酵母质粒对粘蛋白的募集，并导致质粒错配

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The 2μm circle is a highly persistent “selfish” DNA element resident in the Saccharomyces cerevisiae nucleus whose stability approaches that of the chromosomes. The plasmid partitioning system, consisting of two plasmid-encoded proteins, Rep1p and Rep2p, and a cis-acting locus, STB, apparently feeds into the chromosome segregation pathway. The Rep proteins assist the recruitment of the yeast cohesin complex to STB during the S phase, presumably to apportion the replicated plasmid molecules equally to daughter cells. The DNA-protein and protein-protein interactions of the partitioning system, as well as the chromatin organization at STB, are important for cohesin recruitment. Rep1p variants that are incompetent in binding to Rep2p, STB, or both fail to assist the assembly of the cohesin complex at STB and are nonfunctional in plasmid maintenance. Preventing the cohesin-STB association without impeding Rep1p-Rep2p-STB interactions also causes plasmid missegregation. During the yeast cell cycle, the Rep1p and Rep2p proteins are expelled from STB during a short interval between the late G1 and early S phases. This dissociation and reassociation event ensures that cohesin loading at STB is replication dependent and is coordinated with chromosomal cohesin recruitment. In an rsc2Δ yeast strain lacking a specific chromatin remodeling complex and exhibiting a high degree of plasmid loss, neither Rep1p nor the cohesin complex can be recruited to STB. The phenotypes of the Rep1p mutations and of the rsc2Δ mutant are consistent with the role of cohesin in plasmid partitioning being analogous to that in chromosome partitioning.
机译：2μm圆圈是驻留在酿酒酵母细胞核中的高度持久的“自私” DNA元件，其稳定性接近染色体的稳定性。质粒分配系统由两个质粒编码蛋白Rep1p和Rep2p和一个 cis 作用位点 STB 组成，显然进入了染色体分离途径。 Rep蛋白有助于在S期将酵母黏附素复合物募集到 STB ，大概是将复制的质粒分子平均分配给子细胞。分配系统的DNA-蛋白质和蛋白质-蛋白质相互作用以及 STB 处的染色质组织对于凝聚素募集至关重要。无法与Rep2p， STB 或两者结合的Rep1p变体无法协助在 STB 处粘着蛋白复合物的组装，并且在质粒维护中不起作用。在不阻止Rep1p-Rep2p- STB 相互作用的情况下阻止cohesin- STB 缔合也会导致质粒错聚。在酵母细胞周期中，在G 1 晚期和S早期早期之间的短时间间隔内，Rep1p和Rep2p蛋白从 STB 中排出。此解离和重新关联事件可确保 STB 处的粘着蛋白上载是复制依赖性的，并与染色体粘着蛋白募集相协调。在缺乏特定染色质重塑复合物且显示高度质粒丢失的 rsc2 Δ酵母菌株中，Rep1p和粘着蛋白复合物均不能募集到 STB 中。 Rep1p突变和 rsc2 Δ突变体的表型与粘着蛋白在质粒分配中的作用相一致，类似于染色体分配中的作用。

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