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首页> 外文期刊>Molecular and Cellular Biology >Identification of Novel Import and Export Signals of Human TAP, the Protein That Binds to the Constitutive Transport Element of the Type D Retrovirus mRNAs
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Identification of Novel Import and Export Signals of Human TAP, the Protein That Binds to the Constitutive Transport Element of the Type D Retrovirus mRNAs

机译：鉴定新型人类TAP进出口信号，该信号与D型逆转录病毒mRNA的组成型转运元件结合

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摘要

The nuclear export of the unspliced type D retrovirus mRNA depends on the cis-acting constitutive transport RNA element (CTE) that has been shown to interact with the human TAP (hTAP) protein promoting the export of the CTE-containing mRNAs. We report here that hTAP is a 619-amino-acid protein extending the previously identified protein by another 60 residues at the N terminus and that hTAP shares high homology with the predicted rat and mouse TAP proteins. We found that hTAP is a nuclear protein that accumulates in the nuclear rim and the nucleoplasm. We further demonstrated that hTAP is able to shuttle between the nucleus and the cytoplasm. Identification of the signals responsible for nuclear import (NLS) and export (NES) revealed that they are distinct but partially overlapping. NLS and NES of hTAP are active transferable signals that do not share similarities with known elements. The C-terminal portion contributes further to hTAP’s nuclear retention and contains a signal(s) for nuclear rim association. Taken together, our data show that hTAP is a dynamic protein capable of bidirectional trafficking across the nuclear envelope. These data further support hTAP’s role as an export factor of the CTE-containing mRNAs.
机译：未剪接的D型逆转录病毒mRNA的核输出依赖于 cis 作用型组成型转运RNA元件（CTE），该元件已显示与人类TAP（hTAP）蛋白相互作用，从而促进CTE的输出含mRNA。我们在这里报告说，hTAP是一个619个氨基酸的蛋白质，将先前确定的蛋白质在N末端延伸了另外60个残基，并且hTAP与预测的大鼠和小鼠TAP蛋白质具有高度同源性。我们发现，hTAP是一种在核环和核质中积累的核蛋白。我们进一步证明了hTAP能够在细胞核和细胞质之间穿梭。对负责核进口（NLS）和出口（NES）的信号的识别表明，它们是截然不同但部分重叠的。 hTAP的NLS和NES是有效的可传输信号，与已知元素没有相似之处。 C端部分进一步促进hTAP的核固位，并包含一个与核缘结合的信号。综上所述，我们的数据表明，hTAP是一种动态蛋白质，能够跨核膜双向运输。这些数据进一步支持了hTAP作为含有CTE的mRNA的输出因子的作用。

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