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首页> 外文期刊>Molecular and Cellular Biology >Translation of a Small Subset of Caenorhabditis elegans mRNAs Is Dependent on a Specific Eukaryotic Translation Initiation Factor 4E Isoform
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Translation of a Small Subset of Caenorhabditis elegans mRNAs Is Dependent on a Specific Eukaryotic Translation Initiation Factor 4E Isoform

机译：秀丽隐杆线虫小亚型的翻译取决于特定的真核翻译起始因子4E同工型。

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The mRNA cap-binding protein eukaryotic translation initiation factor 4E (eIF4E) participates in protein synthesis initiation, translational repression of specific mRNAs, and nucleocytoplasmic shuttling. Multiple isoforms of eIF4E are expressed in a variety of organisms, but their specific roles are poorly understood. We investigated one Caenorhabditis elegans isoform, IFE-4, which has homologues in plants and mammals. IFE-4::green fluorescent protein (GFP) was expressed in pharyngeal and tail neurons, body wall muscle, spermatheca, and vulva. Knockout of ife-4 by RNA interference (RNAi) or a null mutation produced a pleiotropic phenotype that included egg-laying defects. Sedimentation analysis demonstrated that IFE-4, but not IFE-1, was present in 48S initiation complexes, indicating that it participates in protein synthesis initiation. mRNAs affected by ife-4 knockout were determined by DNA microarray analysis of polysomal distribution. Polysome shifts, in the absence of total mRNA changes, were observed for only 33 of the 18,967 C. elegans mRNAs tested, of which a disproportionate number were related to egg laying and were expressed in neurons and/or muscle. Translational regulation was confirmed by reduced levels of DAF-12, EGL-15, and KIN-29. The functions of these proteins can explain some phenotypes observed in ife-4 knockout mutants. These results indicate that translation of a limited subset of mRNAs is dependent on a specific isoform of eIF4E.
机译：mRNA帽结合蛋白真核翻译起始因子4E（eIF4E）参与蛋白质合成的起始，特定mRNA的翻译抑制和核质穿梭。 eIF4E的多种同工型在多种生物中均有表达，但对其具体作用知之甚少。我们研究了一种秀丽隐杆线虫同种型IFE-4，它在植物和哺乳动物中具有同源性。 IFE-4 ::绿色荧光蛋白（GFP）在咽和尾神经元，体壁肌肉，精囊和外阴中表达。 RNA干扰（RNAi）导致的 ife-4 敲除或无效突变产生了一种多效性表型，其中包括产卵缺陷。沉淀分析表明IFE-4，而不是IFE-1，存在于48S起始复合物中，表明它参与了蛋白质合成的起始。通过多体体分布的DNA微阵列分析确定受 ife-4 敲除影响的mRNA。在18967 C中仅33个观察到多核糖体移位，而mRNA没有变化。秀丽线虫的mRNA，其中不成比例的数量与产卵有关，并在神经元和/或肌肉中表达。通过降低DAF-12，EGL-15和KIN-29的水平来确认翻译调控。这些蛋白质的功能可以解释在 ife-4 敲除突变体中观察到的一些表型。这些结果表明，mRNA的有限子集的翻译取决于eIF4E的特定同工型。

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