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首页> 外文期刊>Molecules >Purified Phlorizin from DocynIa Indica (Wall.) Decne by HSCCC, Compared with Whole Extract, Phlorizin and Non-Phlorizin Fragment Ameliorate Obesity, Insulin Resistance, and Improves Intestinal Barrier Function in High-Fat-Diet-Fed Mice
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Purified Phlorizin from DocynIa Indica (Wall.) Decne by HSCCC, Compared with Whole Extract, Phlorizin and Non-Phlorizin Fragment Ameliorate Obesity, Insulin Resistance, and Improves Intestinal Barrier Function in High-Fat-Diet-Fed Mice

机译：HSCCC提取的来自DocynIa Indica（Wall。）的纯化的萤草素与全提取物相比，萤石素和非萤石素片段可改善肥胖，胰岛素抵抗，并改善高脂饮食喂养小鼠的肠屏障功能

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Natural products generally contain complex and multiple bioactive compounds that areresponsible for the effects on health through complicated synergistic and/or suppressive actions.As an important raw material of local ethnic minority tea, ethnomedicines and food supplementsin southwestern areas of China, Docynia indica (Wall.) Decne (DID) mainly consists of phlorizin(PHZ), which is the main active component. In this study, the holistic activities and the interactionsof components of PHZ, non-phlorizin (NP) in the DID extract (DIDE) were evaluated. A rapidand effective high-speed counter-current chromatography (HSCCC) was performed to knock outPHZ from DIDE and the purity of PHZ was 96.01% determined by HPLC, with a recovery rate of96.76%. After 13 weeks of treatment course in a high-fat diet (HFD)-induced obese mice model,the results revealed that the DIDE and PHZ significantly decreased weight gain, blood lipid levels,hyperplasia of adipocytes and alleviated inflammation (p < 0.05). Both DIDE and PHZ improvesinsulin resistance (p < 0.001). Meanwhile, the intestinal barrier function was improved compared toHFD group, through the determination of serum lipopolysaccharides (LPS), glucagon-likepeptide-2(GLP-2) and hematoxylin-eosin staining of jejunum. Interestingly, after NP treatment, the metabolicsyndrome of the HFD-induced obesity appeared to have a similar improvement. All the experimentsshowed that there is a synergistic weakening phenomenon when PHZ and NP interact with eachother in the mixed state. In conclusion, for the PHZ and NP showing a good effect on anti-obesity,anti-inflammation, and intestinal barrier function, DIDE could be a good source of functional food toprevent obesity.
机译：天然产物通常包含复杂的和多种生物活性化合物，这些化合物通过复杂的协同和/或抑制作用对健康产生影响。作为中国西南地区少数民族茶的重要原料，民族药和食品补充剂多西尼亚in癸烯（DID）主要由phlorizin（PHZ）组成，它是主要的活性成分。在这项研究中，对DID提取物（DIDE）中PHZ，非Phrizrizin（NP）的整体活性及其相互作用进行了评估。进行了快速有效的高速逆流色谱法（HSCCC）从DIDE中敲除PHZ，通过HPLC测定PHZ的纯度为96.01％，回收率为96.76％。在高脂饮食（HFD）诱导的肥胖小鼠模型中治疗13周后，结果显示DIDE和PHZ显着降低了体重增加，血脂水平，脂肪细胞增生和炎症减轻（p <0.05）。 DIDE和PHZ均可改善胰岛素抵抗（p <0.001）。同时，通过测定血清脂多糖（LPS），胰高血糖素样肽-2（GLP-2）和空肠苏木精-伊红染色，与HFD组相比，肠屏障功能得到改善。有趣的是，NP治疗后，HFD诱发的肥胖症的代谢综合征似乎也有类似的改善。所有实验表明，当PHZ和NP在混合状态下相互作用时，存在协同减弱的现象。综上所述，由于PHZ和NP对肥胖，抗炎和肠屏障功能显示出良好的作用，DIDE可能是预防肥胖的功能食品的良好来源。

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