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Electrochemical monitoring of ROS generation by anticancer agents: the case of chartreusin

机译：电化学监测抗癌药产生ROS的情况：黄绿素的情况

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Solution phase and solid-sate electrochemical techniques centered in the voltammetry of microparticles approach are applied for testing the cytotoxic activity of anticancer drugs. The possibility of electrochemical generation of reactive oxygen species (ROS) is exploited for evaluating their contribution to cellular damage. The described methodology is applied to the case of chartreusin (Ch) whose electrochemistry in non-aqueous solutions and in the solid state in contact with aqueous electrolytes is described in the absence (experimental data were confirmed by theoretical calculations) and in the presence of double-stranded DNA (dsDNA). In parallel, scanning electrochemical microscopy (SECM) examination of dsDNA fibers was developed. Electrochemical data suggest that Ch-induced dsDNA interaction can operate by a two pathways: via intercalation and by mechanisms related with ROS generation. Although reduced Ch forms electrochemically generated can act as radical scavengers blocking the ROS generation chain, this property is interrupted upon binding to dsDNA.
机译：以微粒伏安法为中心的溶液相和固相电化学技术被用于测试抗癌药物的细胞毒活性。利用电化学产生活性氧（ROS）的可能性来评估其对细胞损伤的作用。所描述的方法适用于沙特菌素（Ch）的情况，其在非水溶液中（在实验数据已通过理论计算得到证实）和在双倍存在下描述了在非水溶液中以及在与水电解质接触的固态下的电化学链DNA（dsDNA）。并行地，开发了dsDNA纤维的扫描电化学显微镜（SECM）检查。电化学数据表明，Ch诱导的dsDNA相互作用可通过两种途径起作用：通过嵌入和与ROS产生有关的机制。尽管电化学生成的还原的Ch形式可以充当自由基清除剂，从而阻断ROS生成链，但该属性在与dsDNA结合后会中断。

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