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首页> 外文期刊>RSC Advances >UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets
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UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

机译：UHPLC-ESI-Q-TOF-MS / MS分析，抗氧化活性结合指纹图谱评估复方甘草片的质量一致性

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Traditional Chinese medicines (TCM)/herbal medicines (HM) are too complicated to comprehensively investigate their quality consistency effectively with a single detection technique. Hence, finding an effective, rapid, and comprehensive quality control (QC) method is of great importance for guaranteeing the safety and efficacy of TCM/HM in clinical applications. In our current research, a novel strategy of multi-wavelength fusion HPLC fingerprints and ultraviolet (UV) spectroscopic fingerprinting was proposed and successfully applied to monitor the quality consistency of compound liquorice tablets (CLT). The quality grades of 35 CLT samples from two manufacturers were successfully discriminated and evaluated by the averaged linear quantified fingerprint method (ALQFM) from a qualitative and quantitative perspective. The results showed that the UV spectroscopic fingerprints agreed well with the multi-wavelength fusion HPLC fingerprints. In addition, ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-Q-TOF-MS) was applied to investigate the chemical constituents in CLT samples, providing an important chemical structural foundation for further QC and bioactivity studies. Additionally, a simple flow injection analysis (FIA) was developed to investigate the antioxidant capacity in CLT, which was based on the scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals by antioxidants. Furthermore, the fingerprint–efficacy relationship between high-performance liquid chromatography (HPLC) fingerprints and the antioxidant activities of CLT samples was established utilizing orthogonal projection to latent structures (OPLS). In conclusion, this study indicated that integrating UHPLC-ESI-Q-TOF-MS/MS, UV spectroscopic fingerprints, and multi-wavelength fusion HPLC fingerprints coupled with the antioxidant activities reported could give important clues for further pharmacological and clinical studies of CLT. Meanwhile, it provides a practical strategy for the rapid screening and identifying of TCM/HM quality consistency.
机译：传统中药（TCM）/草药（HM）太复杂，无法通过一种检测技术有效地全面研究其质量一致性。因此，寻找有效，快速，全面的质量控制（QC）方法对于保证TCM / HM在临床应用中的安全性和有效性至关重要。在我们目前的研究中，提出了一种多波长融合HPLC指纹图谱和紫外（UV）光谱指纹图谱的新策略，并将其成功地用于监测复方甘草片（CLT）的质量一致性。从定性和定量的角度，通过平均线性定量指纹法（ALQFM）成功区分和评估了两个制造商的35个CLT样品的质量等级。结果表明，紫外光谱指纹图谱与多波长融合HPLC指纹图谱吻合良好。此外，超高效液相色谱结合电喷雾电离四极杆飞行时间质谱（UHPLC-ESI-Q-TOF-MS）用于研究CLT样品中的化学成分，提供了重要的化学结构基础用于进一步的质量控制和生物活性研究。此外，开发了一种简单的流动注射分析（FIA），以研究CLT中的抗氧化剂能力，该方法基于抗氧化剂清除2,2-二苯基-1-吡啶并肼基的作用。此外，利用对潜在结构（OPLS）的正交投影建立了高效液相色谱（HPLC）指纹与CLT样品的抗氧化活性之间的指纹功效关系。总之，这项研究表明，将UHPLC-ESI-Q-TOF-MS / MS，UV光谱指纹图谱和多波长融合HPLC指纹图谱结合起来，再加上报道的抗氧化活性，可以为进一步CLT的药理和临床研究提供重要线索。同时，为中药/中药质量一致性的快速筛选和鉴定提供了实用的策略。

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