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首页> 外文期刊>RSC Advances >The effects of macromolecular crowding and surface charge on the properties of an immobilized enzyme: activity, thermal stability, catalytic efficiency and reusability
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The effects of macromolecular crowding and surface charge on the properties of an immobilized enzyme: activity, thermal stability, catalytic efficiency and reusability

机译：大分子拥挤和表面电荷对固定化酶性质的影响：活性，热稳定性，催化效率和可重复使用性

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The microenvironment around an immobilized enzyme molecule significantly influences the properties of the immobilized enzyme. To explore the combined effects of macromolecular crowding and surface charge on the properties of an immobilized enzyme, epoxy resin was modified by different quality percentage concentrations of chitosan to obtain different crowding and charge conditions of the microenvironment. With this system, the catalytic features of immobilized 7α- and 7β-hydroxysteroid dehydrogenase (7α-, 7β-HSDH) were investigated. The results indicate that the activities and thermal stabilities of immobilized 7α-HSDH and 7β-HSDH can be improved by manipulating the crowding and surface charge effects. The relative activity of immobilized 7α-HSDH and 7β-HSDH on EP-0.5-C increased approximately 90% and 50% relative to the immobilization onto EP. The immobilization of 7α-HSDH and 7β-HSDH onto EP-0.5-C preserved a higher activity (approximately 73% and 88% of the initial activity) than that preserved by immobilization onto EP (approximately 29% and 40% of the initial activity) after incubation at 45 °C for 2 h. In addition, the catalytic efficiency and reusability of co-immobilized 7α-HSDH and 7β-HSDH was improved. The TCDCA conversion of the co-immobilized enzymes onto EP-0.5-C was increased approximately 45%, compared to the conversion of the co-immobilized enzymes onto EP. The TCDCA conversion of the co-immobilized enzymes on EP-0.5-C remained at 83.72 ± 0.66% after seven successive cycles. These findings suggest that crowding and charge of the microenvironment can significantly improve the properties of the immobilized enzyme.
机译：固定化酶分子周围的微环境会显着影响固定化酶的性质。为了探索大分子拥挤和表面电荷对固定化酶性能的综合影响，通过不同质量百分比浓度的壳聚糖对环氧树脂进行改性，以获得不同的微环境拥挤和电荷条件。利用该系统，研究了固定化的7α-和7β-羟基类固醇脱氢酶（7α-，7β-HSDH）的催化特性。结果表明，固定的7α-HSDH和7β-HSDH的活性和热稳定性可以通过控制拥挤和表面电荷效应来提高。相对于固定在EP上，固定的7α-HSDH和7β-HSDH在EP-0.5-C上的相对活性增加了约90％和50％。将7α-HSDH和7β-HSDH固定在EP-0.5-C上的活性比固定在EP上的活性高（分别约为初始活性的29％和40％），保留的活性更高（约为初始活性的73％和88％）。 ）在45°C下孵育2小时后。此外，共固定的7α-HSDH和7β-HSDH的催化效率和可重复使用性得到改善。与共固定的酶到EP上的转化相比，共固定的酶到EP-0.5-C上的TCDCA转化增加了约45％。在七个连续的循环后，共固定化酶在EP-0.5-C上的TCDCA转化率保持在83.72±0.66％。这些发现表明，微环境的拥挤和充电可以显着改善固定化酶的特性。

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