• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >In silico analysis and in vivo tests of the tuna dark muscle hydrolysate anti-oxidation effect
【24h】

In silico analysis and in vivo tests of the tuna dark muscle hydrolysate anti-oxidation effect

机译：金枪鱼黑肌肉水解物抗氧化作用的计算机分析和体内试验

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Hydrolysate is a mixture of various peptides with specific functions. However, functional identification of hydrolysate with high throughput is still a difficult task. Furthermore, using in vivo tests via animal or cell experiments is time and labor-intensive. In this study, the peptides component of hydrolysate derived from the tuna dark muscle was measured via MALDI-TOF/TOF-MS, and the functions of the KEFT (Lys-Glu-Phe-Thr), EEASA (Glu-Glu-Ala-Ser-Ala) and RYDD (Arg-Tyr-Asp-Asp) peptides, which were found with the highest proportion, were predicted via Discovery Studio 2016 software. All three peptides were predicted to bind to the Keap1 protein with the highest fit-value and to affect the activity of Keap1, which is involved in anti-oxidation pathways. Subsequently, mice experiments showed that administration of tuna dark muscle hydrolysate increased the levels of superoxide dismutase and glutathione peroxidase in the serum and liver ( P < 0.05) and decreased the malondialdehyde level ( P < 0.05) as well as transcription of Keap1 ( P > 0.05), which are consistent with the in silico analysis results using Discovery Studio 2016 software. The combination of in silico analysis and in vivo tests provided an alternative strategy for identifying hydrolysate function and provided insight into high-value utilization of protein hydrolysate.
机译：水解产物是具有特定功能的各种肽的混合物。然而，高通量的水解产物的功能鉴定仍然是困难的任务。此外，通过动物或细胞实验进行体内测试是费时费力的。在这项研究中，通过MALDI-TOF / TOF-MS测定了来自金枪鱼黑肌肉的水解产物的肽成分，并测定了KEFT（Lys-Glu-Phe-Thr），EEASA（Glu-Glu-Ala-通过Discovery Studio 2016软件预测了比例最高的Ser-Ala）和RYDD（Arg-Tyr-Asp-Asp）肽。预计所有这三种肽都将以最高的拟合值与Keap1蛋白结合并影响Keap1的活性，而Keap1参与了抗氧化途径。随后，小鼠实验表明，使用金枪鱼黑肌肉水解产物可增加血清和肝脏中的超氧化物歧化酶和谷胱甘肽过氧化物酶水平（P <0.05），并降低丙二醛水平（P <0.05）以及Keap1的转录（P> 0.05），与使用Discovery Studio 2016软件进行的计算机分析结果一致。计算机分析和体内测试的结合提供了一种识别水解产物功能的替代策略，并提供了对蛋白质水解产物高价值利用的洞察力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号