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In silico analysis and in vivo tests of the tuna dark muscle hydrolysate anti-oxidation effect

机译：在硅分析和金枪鱼黑肌的体内试验中水解抗氧化效果

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摘要

Hydrolysate is a mixture of various peptides with specific functions. However, functional identification of hydrolysate with high throughput is still a difficult task. Furthermore, using in vivo tests via animal or cell experiments is time and labor-intensive. In this study, the peptides component of hydrolysate derived from the tuna dark muscle was measured via MALDI-TOF/TOF-MS, and the functions of the KEFT (Lys-Glu-Phe-Thr), EEASA (Glu-Glu-Ala-Ser-Ala) and RYDD (Arg-Tyr-Asp-Asp) peptides, which were found with the highest proportion, were predicted via Discovery Studio 2016 software. All three peptides were predicted to bind to the Keap1 protein with the highest fit-value and to affect the activity of Keap1, which is involved in anti-oxidation pathways. Subsequently, mice experiments showed that administration of tuna dark muscle hydrolysate increased the levels of superoxide dismutase and glutathione peroxidase in the serum and liver (P 0.05) and decreased the malondialdehyde level (P 0.05) as well as transcription of Keap1 (P 0.05), which are consistent with the in silico analysis results using Discovery Studio 2016 software. The combination of in silico analysis and in vivo tests provided an alternative strategy for identifying hydrolysate function and provided insight into high-value utilization of protein hydrolysate.
机译：水解产物是具有特定功能的各种肽的混合物。然而，具有高吞吐量的水解产物的功能识别仍然是一项艰巨的任务。此外，通过动物或细胞实验使用体内试验是时间和劳动密集型。在这项研究中，通过MALDI-TOF / TOF-MS测量源自金枪鱼暗肌的水解产物的肽组分，以及KEFT（LYS-GLU-PHE-THR），EEASA（Glu-glu-Ala-通过Discovery Studio 2016软件预测，以最高比例发现的Ser-Ala）和Rydd（Arg-Tyr-ASP-ASP）肽。预计所有三种肽与最高的抗氧化途径参与抗氧化途径的keap1蛋白质与最高的keap1蛋白质结合。随后，小鼠实验表明，金枪鱼暗肌水解产物的给药增加了血清和肝脏中超氧化物歧化酶和谷胱甘肽过氧化物酶的水平（P <0.05），并降低了丙醛水平（P <0.05）以及Keap1的转录（ p＆ 0.05），它与使用Discovery Studio 2016软件的Silico分析结果一致。在硅分析和体内试验中的组合提供了一种替代策略，用于识别水解产物功能，并提供对蛋白质水解产物的高价值使用的洞察力。

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  • 来源
    《RSC Advances》 |2018年第25期|共11页
  • 作者

    Han Jiaojiao; Tang Shasha; Li Yanyan; Bao Wei; Wan Haitao; Lu Chenyang; Zhou Jun; Li Ye; Cheong Lingzhi; Su Xiurong;

  • 作者单位

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Cornell Univ Coll Agr &

    Life Sci Ithaca NY 14853 USA;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

    Ningbo Univ Sch Marine Sci 818 Fenghua Rd Ningbo Zhejiang Peoples R China;

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