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首页> 外文期刊>RSC Advances >Acidolysis-dominated pretreatment elevates distillation yield and impacts composition, antioxidant and antifungal activities of essential oil from Cuminum cyminum seeds
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Acidolysis-dominated pretreatment elevates distillation yield and impacts composition, antioxidant and antifungal activities of essential oil from Cuminum cyminum seeds

机译：酸解为主的预处理可提高蒸馏产率，并影响小茴香种子精油的成分，抗氧化剂和抗真菌活性

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Proper pretreatment of herbal material containing essential oils (EOs) could enhance its volatile components release through either removing physical barriers or conquering chemical bonds and thereby improve hydrodistillation yield. In this regard, a trial pretreatment including pulverization, enzymolysis, short time microwave irradiation and acidolysis of Cuminum cyminum seeds was integrated into the essential oil (EO) preparation to elevate the EO yield. On the basis of Plackett–Burman design analysis, three parameters (acidolysis duration, HCl concentration of acidolysis and sieving mesh) were significant for the EO preparation. Box–Behnken design based optimization of the remaining factors concluded that the optimal pretreatment was pulverizing the seeds to 40 mesh and implementing 45 min acidolysis in 2.5 M L ~(?1) HCl wherein the predicted EO yield of 3.78% was close to that of the experimental value 3.86%. This pretreatment produced an EO yield increase of 50.78% over the control sample of raw seeds (2.56%). In total 53 components were identified in the acidolysis-pretreated cumin EO (AEO) whilst 47 components were identified in the control cumin EO (CEO). In both AEO and CEO, cuminaldehyde was the predominant common component, but the AEO contained more phenols (0.51% vs. 0.18%) and alcohols (7.76% vs. 0.18%) than the CEO did. The compositional features gave the AEO mightier antioxidant potency and stronger antifungal efficacy against four postharvest fungi, viz. Alternaria alternata , Penicillium expansum , Trichothecium roseum and Fusarium sulphureum , as compared with the situations of CEO. In conclusion, the pretreatment elevates the hydrodistillation yield, modifies the EO chemical profiles and confers stronger antioxidant and antifungal activities upon cumin EO.
机译：适当预处理含有精油（EOs）的草药材料可以通过消除物理障碍或克服化学键来提高其挥发性成分的释放，从而提高加氢蒸馏的产量。在这方面，将包括粉碎，酶解，短时间微波辐射和小茴香种子酸解的试验性预处理整合到香精油（EO）制剂中，以提高EO产量。根据Plackett-Burman设计分析，三个参数（酸解持续时间，酸解的HCl浓度和筛目）对于EO制备很重要。基于Box–Behnken设计对其余因素进行优化的结论是，最佳的预处理方法是将种子粉碎成40目，并在2.5 ML〜（？1）HCl中进行45分钟酸解，其中预测的EO收率为3.78％。实验值3.86％。与原始种子的对照样品（2.56％）相比，这种预处理使EO产量提高了50.8％。在酸解预处理的孜然EO（AEO）中总共鉴定出53种成分，而在对照孜然EO（CEO）中鉴定出47种成分。在AEO和CEO中，孜然醛是主要的共同成分，但是AEO所含的酚（0.51％比0.18％）和酒精（7.76％比0.18％）要比CEO多。成分特征使AEO的抗氧化能力更强，并且对四种采后真菌也具有更强的抗真菌功效。与首席执行官的情况相比，链格孢菌，扩展青霉，玫瑰毛癣菌和硫镰刀菌。总之，该预处理提高了水蒸馏的产率，改变了EO的化学特性，并赋予了孜然EO以更强的抗氧化和抗真菌活性。

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