Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp

Benjarat Bunterngsook; Thanaporn Laothanachareon; Suda Natrchalayuth; Sirithorn Lertphanich; Tatsuya Fujii; Hiroyuki Inoue; Chalermkiart Youngthong; Duriya Chantasingh; Lily Eurwilaichitr; Verawat Champreda

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Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp

机译：木薯原浆水解最小协同酶系统的优化

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Cassava pulp is an underused agricultural by-product comprising residual starch granules entrapped in cell wall polysaccharides, making it unique from other lignocellulosic wastes in terms of enzymatic processing. In this study, a synergistic system comprising a minimal set of enzymes tailor-made for efficient bioprocessing of raw cassava pulp was developed. The fiber-degrading enzyme mixture comprises an endoglucanase (Cel12), β-glucosidase (BGL), endo-β-1,4-xylanase (XYN) and endo-polygalacturonase (EPG) from Aspergillus aculeatus and a cellobiohydrolase I (Cel7A) from Talaromyces cellulolyticus was initially optimized using the experimental design approach. A glucose recovery yield of 91.3% based on the total starch and cellulose content was obtained from saccharification of cassava pulp using the combination of 5.0 mg g?1 of fiber degrading enzyme mixture comprising Cel12, Cel7A, BGL, XYN and EPG in the ratio of 16.5?:?25.5?:?15.0?:?18.0?:?25.0 together with 0.5 mg g?1 of raw starch degrading enzyme Stargen? 002 with the degree of synergy of 1.43. Efficient hydrolysis was achieved without an energy-intensive pretreatment step, showing industrial applicability on saccharification and modification of cassava pulp and for further incorporation of these enzymes into ethanologens for consolidated bioprocessing.

机译：木薯纸浆是一种未充分利用的农业副产品，包含残留在细胞壁多糖中的残留淀粉颗粒，在酶促加工方面使其不同于其他木质纤维素废料。在这项研究中，开发了一种协同系统，该系统包含最少量的酶，这些酶量身定制，可对木薯原浆进行有效的生物加工。纤维降解酶混合物包含来自 aculgus aculeatus 的内切葡聚糖酶（Cel12），β-葡萄糖苷酶（BGL），内切β-1,4-木聚糖酶（XYN）和内聚半乳糖醛酸酶（EPG），以及首先使用实验设计方法优化了来自 Talaromyces cellulolyticus 的纤维二糖水解酶I（Cel7A）。木薯浆糖化使用5.0 mg g ？1 纤维降解酶混合物组合糖化后，基于总淀粉和纤维素含量的葡萄糖回收率为91.3％包含比例为16.5？：？25.5？：？15.0？：？18.0？：？25.0的Cel12，Cel7A，BGL，XYN和EPG以及0.5 mg g ？1 002，协同度为1.43。无需进行能源密集的预处理步骤即可实现有效的水解，显示出对木薯纸浆的糖化和改性以及将这些酶进一步掺入乙醇原中以进行整合生物处理的工业适用性。 展开▼

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《RSC Advances》 |2017年第76期|共10页

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Benjarat Bunterngsook; Thanaporn Laothanachareon; Suda Natrchalayuth; Sirithorn Lertphanich; Tatsuya Fujii; Hiroyuki Inoue; Chalermkiart Youngthong; Duriya Chantasingh; Lily Eurwilaichitr; Verawat Champreda;
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1. Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp [J] . Bunterngsook Benjarat, Laothanachareon Thanaporn, Natrchalayuth Suda, RSC Advances . 2017,第76期

机译：优化原料木薯纸浆水解最小协同酶系统

2. Optimisation of synergistic biomass-degrading enzyme systems for efficient rice straw hydrolysis using an experimental mixture design [J] . Suwannarangsee S., Bunterngsook B., Arnthong J., Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies . 2012,第Null期

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3. A Comparison of the Production of Ethanol between Simultaneous Sacchariflcation and Fermentation and Separate Hydrolysis and Fermentation Using Unpretreated Cassava Pulp and Enzyme Cocktail [J] . Mingjun Zhu, Ping Li, Xinfang Gong Bioscience, Biotechnology, and Biochemistry . 2012,第4期

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6. Optimization of a synthetic mixture composed of major Trichoderma reesei enzymes for the hydrolysis of steam-exploded wheat straw [O] . Hélène Billard, Abdelaziz Faraj, Nicolas Lopes Ferreira, 2012

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8. Hydrolysis of solubilized hemicellulose derived from wet-oxidized wheat straw by a mixture of commercial fungal enzyme preparations [R] . Skammelsen Schmidt, A. , Thomsen, A. B. , Woidemann, A. , 1998

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4. 木薯淀粉基环保型木材胶粘剂的合成研究（Ⅱ）--基于淀粉水解液改性脲醛树脂的配方优化 [J] . 潘礼成 ,甘卫星 ,莫德旺 . 桂林理工大学学报 . 2014,第001期

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6. 木薯渣水解液中主要发酵抑制物分析研究 [C] . XU Ting-ting ,徐婷婷 ,TIAN Hua-li . 中国造纸学会第十七届学术年会 . 2016

7. 木薯渣水解液发酵产乙醇的工艺优化及代谢流量分析研究 [A] . 邓永东 . 2018

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4. HYDROLYSIS OF SHELL AND CASSAVA OF CASSAVA USING AMYLOLYTIC ENZYMES PRODUCED BY FUNGUS [P] . 外国专利： BR102018073305A2 . 2020-06-02

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机译：分析生物质水解过程中纤维素材料水解过程中纤维素降解的方法以及木质纤维素的水解过程，确定目标酶是否影响纤维素水解的方法，高效率分析酶和/或的方法感兴趣的建筑物，用于分析酶系统性能以评估酶性能的方法，用于确定纤维素量的归一化方法，生物样品的目标，用于归一化荧光强度数据的方法和方法用于确定目的酶和目的多肽是否影响纤维素的水解

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Optimization of a minimal synergistic enzyme system for hydrolysis of raw cassava pulp

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