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首页> 外文期刊>RSC Advances >A multifunctional oxidosqualene cyclase from Tripterygium regelii that produces both α- and β-amyrin
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A multifunctional oxidosqualene cyclase from Tripterygium regelii that produces both α- and β-amyrin

机译：雷公藤的多功能氧化角鲨烯环化酶，同时产生α-和β-糊精

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摘要

Tripterygium regelii is a rich source of triterpenoids, containing many types of triterpenes with high chemical diversity and interesting pharmacological properties. The cDNA of the multifunctional oxidosqualene cyclase ( TrOSC , GenBank accession number: MH161182), consisting of a 2289 bp open reading frame and coding for 762 amino acids, was cloned from the stems and roots of Tripterygium regelii . Phylogenetic analysis using OSC genes from other plants suggested that TrOSC might be a mixed-amyrin synthase. The coding sequence was cloned into the expression vector pYES2 and transformed into the yeast Saccharomyces cerevisiae . The resulting products were analysed by GC-MS. Surprisingly, although it showed 76% sequence identity to lupeol synthase from Ricinus communis , TrOSC was found to be a multifunctional triterpene synthase producing both α- and β-amyrin, the precursors of ursane and oleanane type triterpenes, respectively. qRT-PCR analysis revealed that the transcript of TrOSC accumulated mainly in roots and stems. Taken together, our findings contribute to the knowledge of key genes in the pentacyclic triterpene biosynthesis pathway.
机译：雷公藤雷公藤是三萜类化合物的丰富来源，其中包含许多类型的三萜类化合物，具有高度的化学多样性和令人感兴趣的药理特性。从雷公藤的茎和根中克隆了多功能氧化角鲨烯环化酶（TrOSC，GenBank登录号：MH161182）的cDNA，该cDNA由2289 bp的开放阅读框组成，编码762个氨基酸。使用来自其他植物的OSC基因进行的系统发育分析表明，TrOSC可能是混合的amyrin合酶。将编码序列克隆到表达载体pYES2中，并转化到酵母酿酒酵母（Saccharomyces cerevisiae）中。通过GC-MS分析所得产物。出人意料的是，尽管它与来自蓖麻的羽扇豆合酶显示76％的序列同一性，但发现TrOSC是一种多功能的三萜合酶，可同时产生α-和β-淀粉糊精，分别是乌烷和乌拉烷型三萜的前体。 qRT-PCR分析表明，TrOSC的转录本主要在根和茎中积累。两者合计，我们的发现有助于五环三萜生物合成途径中的关键基因的知识。

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