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首页> 外文期刊>RSC Advances >A multifunctional oxidosqualene cyclase from Tripterygium regelii that produces both alpha- and beta-amyrin
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A multifunctional oxidosqualene cyclase from Tripterygium regelii that produces both alpha- and beta-amyrin

机译：来自初学者雷格西的多官能氧化喹啉环酶，其产生α-和β-氨基

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摘要

Tripterygium regelii is a rich source of triterpenoids, containing many types of triterpenes with high chemical diversity and interesting pharmacological properties. The cDNA of the multifunctional oxidosqualene cyclase (TrOSC, GenBank accession number: MH161182), consisting of a 2289 bp open reading frame and coding for 762 amino acids, was cloned from the stems and roots of Tripterygium regelii. Phylogenetic analysis using OSC genes from other plants suggested that TrOSC might be a mixed-amyrin synthase. The coding sequence was cloned into the expression vector pYES2 and transformed into the yeast Saccharomyces cerevisiae. The resulting products were analysed by GC-MS. Surprisingly, although it showed 76% sequence identity to lupeol synthase from Ricinus communis, TrOSC was found to be a multifunctional triterpene synthase producing both - and -amyrin, the precursors of ursane and oleanane type triterpenes, respectively. qRT-PCR analysis revealed that the transcript of TrOSC accumulated mainly in roots and stems. Taken together, our findings contribute to the knowledge of key genes in the pentacyclic triterpene biosynthesis pathway.
机译：Tripterygium Regelii是一个丰富的三萜来源，包含许多具有高化学多样性和有趣药理学性质的三种三萜。由2289bp开放阅读框架和762个氨基酸编码组成的多官能氧化喹吖啶环酶（TROSC，Genbank登录号：MH161182）的cDNA，从TRETYGIUM Regelii的茎和根部克隆了762个氨基酸。使用来自其他植物的OSC基因的系统发育分析表明TROSC可能是混合氨基合酶。将编码序列克隆到表达载体pyes2中并转化到酵母酿酒酵母中。通过GC-MS分析所得产物。令人惊讶的是，虽然它显示了来自Ricinus Communis的Lupeol合酶的76％序列同一性，发现TROSC分别是多官能三萜合酶，分别产生 - 和-Myrin，甲烷和烯烃型三萜的前体。 QRT-PCR分析表明，TROSC的转录物主要累积在根和茎中。我们的发现在一起，有助于对五环素三萜生物合成途径中的关键基因的知识。

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  • 来源
    《RSC Advances》 |2018年第42期|共6页
  • 作者

    Lu Yun; Zhou Jiawei; Hu Tianyuan; Zhang Yifeng; Su Ping; Wang Jiadian; Gao Wei; Huang Luqi;

  • 作者单位

    Capital Med Univ Sch Tradit Chinese Med Beijing 100069 Peoples R China;

    Capital Med Univ Sch Tradit Chinese Med Beijing 100069 Peoples R China;

    Capital Med Univ Sch Tradit Chinese Med Beijing 100069 Peoples R China;

    Capital Med Univ Sch Tradit Chinese Med Beijing 100069 Peoples R China;

    China Acad ChineseMed Sci Natl Resource Ctr Chinese MateriaMed State Key Lab Daodi Herbs Beijing Peoples R China;

    Capital Med Univ Sch Tradit Chinese Med Beijing 100069 Peoples R China;

    Capital Med Univ Sch Tradit Chinese Med Beijing 100069 Peoples R China;

    China Acad ChineseMed Sci Natl Resource Ctr Chinese MateriaMed State Key Lab Daodi Herbs Beijing Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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