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首页> 外文期刊>RSC Advances >Bioassay-guided isolation and identification of anticancer and antioxidant compounds from Gynostemma pentaphyllum (Thunb.) Makino
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Bioassay-guided isolation and identification of anticancer and antioxidant compounds from Gynostemma pentaphyllum (Thunb.) Makino

机译：生物测定指导下的绞股蓝绞股蓝抗癌和抗氧化化合物的分离和鉴定

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Gynostemma pentaphyllum (Thunb.) Makino is a medicinal and edible plant in China whose buds and leaves are used for making a popular kind of tea drink. The anticancer and antioxidant properties of the ethyl acetate (EA) and n -butanol ( n -Bu) fractions provide a basis for conducting experiments for isolation and identification of key compounds that may be responsible for the aforementioned properties of G. pentaphyllum . Four compounds were isolated from the two fractions using ODS packing column, silica gel column, polyamide column, Sephadex LH-20 gel column and HPLC. With the aid of ~(1) H, ~(13) C NMR and mass spectrometry, they were identified as 3,4-dihydroxy phenyl- O -β- D -glucoside, gypenoside XLVI, gypenoside L and ginsenoside Rd. 3,4-Dihydroxy phenyl- O -β- D -glucoside showed the strongest DPPH (97.23%) and ABTS (101.37%) scavenging effect and ferric ion reducing power (FRAP value 0.8846), which may be closely related to the hydrogen atoms of phenolic hydroxyls. Gypenoside L and ginsenoside Rd displayed the highest inhibition of tumor cell proliferation of A549 and MCF-7 cell lines, which had to do with the chemical structure of the compounds bearing glycosylated parts and free hydroxyls at the 20th or 21st carbon atom of dammarane-type saponin.
机译：绞股蓝绞股蓝（Thunb。）Makino是中国的药用和食用植物，其芽和叶被用来制成一种流行的茶饮料。乙酸乙酯（EA）和正丁醇（n-Bu）馏分的抗癌和抗氧化特性为进行分离和鉴定可能导致上述五子花的上述特性的关键化合物进行实验提供了基础。使用ODS填充柱，硅胶柱，聚酰胺柱，Sephadex LH-20凝胶柱和HPLC从这两个馏分中分离出四种化合物。借助〜（1）H，〜（13）NMR和质谱，将它们鉴定为3,4-二羟基苯基-O-β-D-葡萄糖苷，人参皂苷XLVI，人参皂苷L和人参皂苷Rd。 3,4-二羟基苯基-O-β-D-葡萄糖苷显示出最强的DPPH清除作用和ABTS清除作用（101.37％）和三价铁离子还原能力（FRAP值0.8846），这可能与氢原子密切相关酚羟基。绞股蓝皂甙L和人参皂苷Rd对A549和MCF-7细胞系的肿瘤细胞增殖表现出最高的抑制作用，这与丹马烷型20或21位碳原子上带有糖基化部分和游离羟基的化合物的化学结构有关皂素。

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