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首页> 外文期刊>RSC Advances >Bioassay-guided isolation and identification of anticancer and antioxidant compounds from Gynostemma pentaphyllum (Thunb.) Makino
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Bioassay-guided isolation and identification of anticancer and antioxidant compounds from Gynostemma pentaphyllum (Thunb.) Makino

机译：生物测定引导和抗氧化和抗氧化剂的分离和鉴定来自颅骨脑枢轴（THUNB.）MAKINO

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摘要

Gynostemma pentaphyllum (Thunb.) Makino is a medicinal and edible plant in China whose buds and leaves are used for making a popular kind of tea drink. The anticancer and antioxidant properties of the ethyl acetate (EA) and n-butanol (n-Bu) fractions provide a basis for conducting experiments for isolation and identification of key compounds that may be responsible for the aforementioned properties of G. pentaphyllum. Four compounds were isolated from the two fractions using ODS packing column, silica gel column, polyamide column, Sephadex LH-20 gel column and HPLC. With the aid of H-1, C-13 NMR and mass spectrometry, they were identified as 3,4-dihydroxy phenyl-O--d-glucoside, gypenoside XLVI, gypenoside L and ginsenoside Rd. 3,4-Dihydroxy phenyl-O--d-glucoside showed the strongest DPPH (97.23%) and ABTS (101.37%) scavenging effect and ferric ion reducing power (FRAP value 0.8846), which may be closely related to the hydrogen atoms of phenolic hydroxyls. Gypenoside L and ginsenoside Rd displayed the highest inhibition of tumor cell proliferation of A549 and MCF-7 cell lines, which had to do with the chemical structure of the compounds bearing glycosylated parts and free hydroxyls at the 20th or 21st carbon atom of dammarane-type saponin.
机译：Gynostemma pentaphyllum（Thunb。）Makino是中国的药用和食用植物，其芽和叶子用于制作一种流行的茶饮。乙酸乙酯（EA）和正丁醇（N-BU）级分的抗癌和抗氧化性质为进行实验提供了用于分离和鉴定可能是负责的前述G.Pentaphyllum的关键化合物的实验的基础。使用ODS包装柱，硅胶柱，聚酰胺柱，Sephadex LH-20凝胶柱和HPLC从两个级分中分离出四种化合物。借助于H-1，C-13 NMR和质谱法，将它们鉴定为3,4-二羟基苯基-O-D-葡糖苷，石奈糖苷XLVI，吉尼诺苷L和人参皂苷Rd。 3,4-二羟基苯基-O - D-葡糖苷显示出最强的DPPH（97.23％）和ABTS（101.37％）清除效果和铁离子还原功率（FRAP值0.8846），其可能与氢原子密切相关酚醛羟基。基因钠L和人参皂甙RD显示出A549和MCF-7细胞系的肿瘤细胞增殖的最高抑制，其与含有糖基化的含量的化合物的化学结构和达摩亚型20th碳原子中的含有糖基化的零件和游离羟基的化学结构有关皂苷。

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  • 来源
    《RSC Advances》 |2018年第41期|共10页
  • 作者

    Wang Tian-Xing; Shi Man-Man; Jiang Jian-Guo;

  • 作者单位

    South China Univ Technol Coll Food &

    Bioengn Guangzhou 510640 Guangdong Peoples R China;

    South China Univ Technol Coll Food &

    Bioengn Guangzhou 510640 Guangdong Peoples R China;

    South China Univ Technol Coll Food &

    Bioengn Guangzhou 510640 Guangdong Peoples R China;

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  • 正文语种 eng
  • 中图分类 化学;
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