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Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro

机译：膜模拟聚合物和肽的微模式固定用于调节体外细胞行为

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To regulate the behaviors and functions of endothelial cells (ECs) on the biomaterials on titanium (Ti), a biomimetic micropattern (ridge/groove: 25/25 μm) of polymer of 2-methacryloyloxyethyl phosphorylcholine (polyMPC) and Gly-Arg-Glu-Asp-Val-Tyr (GREDVY) was fabricated. PMMPC (monomer contain MPC and methacrylic acid (MA)) containing carboxyl groups was chosen, and PMMPC was cross-linked with hexamethylene diamine through condensation reaction of amino and carboxyl. Simultaneously, the carboxyl groups of cross-linked PMMPC (PMMPC-HD) can react with amino groups of polydopamine which can adhered on many materials firmly. GREDVY was immobilized on polydopamine but not on PMMPC-HD because amino and carboxyl groups can react with catechol and amino groups of polydopamine. IR and ~(1) H NMR demonstrated that PMMPC-HD was successfully synthesized. And the QCM-D (quartz crystal microbalance with dissipation) and IR approved that PMMPC-HD and GREDVY can be immobilized on polydopamine (PDA). Platelet adhesion and whole blood adhesion on micropattern modificated with PMMPC and GREDVY (Ti-PDA-M/R(P)) showed better hemocompatibility than other samples. Endothelial cells were regulated in the direction of micropattern showing elongated ECs were closer to a healthy, athero-protective phenotype than ECs cultured in vitro without micropattern. NO and PGI _(2) release were upregulated. Simultaneously the number of SMCs on Ti-PDA-M/R(P) was the smaller that of other samples, which demonstrated that the Ti-PDA-M/R(P) had property of inhibiting SMCs proliferation to a certain extent.
机译：为了调节内皮细胞（EC）在钛（Ti）上的生物材料上的行为和功能，是由2-甲基丙烯酰氧基乙基磷酰胆碱（polyMPC）和Gly-Arg-Glu聚合物组成的仿生微图案（脊/槽：25/25μm） -Asp-Val-Tyr（GREDVY）被制造。选择含有羧基的PMMPC（含有MPC和甲基丙烯酸（MA）的单体），通过氨基与羧基的缩合反应使PMMPC与六亚甲基二胺交联。同时，交联的PMMPC（PMMPC-HD）的羧基可以与聚多巴胺的氨基发生反应，而聚多巴胺可以牢固地粘附在许多材料上。 GREDVY固定在聚多巴胺上而不固定在PMMPC-HD上，因为氨基和羧基可以与邻苯二酚和聚多巴胺的氨基反应。 IR和〜（1）H NMR证明成功地合成了PMMPC-HD。 QCM-D（带耗散的石英晶体微天平）和IR证明PMMPC-HD和GREDVY可以固定在聚多巴胺（PDA）上。在用PMMPC和GREDVY（Ti-PDA-M / R（P））修饰的微模式上，血小板粘附和全血粘附表现出比其他样品更好的血液相容性。内皮细胞在微模式的方向上受到调节，显示出伸长的EC比没有微模式的体外培养的EC更接近健康的，具有动脉粥样硬化保护的表型。 NO和PGI_（2）释放被上调。同时，Ti-PDA-M / R（P）上SMC的数量较少，这说明Ti-PDA-M / R（P）具有一定程度的抑制SMC增殖的特性。

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