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Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro

机译：膜模拟聚合物和肽的微透明理由固定化，用于体外调节细胞行为

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摘要

To regulate the behaviors and functions of endothelial cells (ECs) on the biomaterials on titanium (Ti), a biomimetic micropattern (ridge/groove: 25/25 m) of polymer of 2-methacryloyloxyethyl phosphorylcholine (polyMPC) and Gly-Arg-Glu-Asp-Val-Tyr (GREDVY) was fabricated. PMMPC (monomer contain MPC and methacrylic acid (MA)) containing carboxyl groups was chosen, and PMMPC was cross-linked with hexamethylene diamine through condensation reaction of amino and carboxyl. Simultaneously, the carboxyl groups of cross-linked PMMPC (PMMPC-HD) can react with amino groups of polydopamine which can adhered on many materials firmly. GREDVY was immobilized on polydopamine but not on PMMPC-HD because amino and carboxyl groups can react with catechol and amino groups of polydopamine. IR and H-1 NMR demonstrated that PMMPC-HD was successfully synthesized. And the QCM-D (quartz crystal microbalance with dissipation) and IR approved that PMMPC-HD and GREDVY can be immobilized on polydopamine (PDA). Platelet adhesion and whole blood adhesion on micropattern modificated with PMMPC and GREDVY (Ti-PDA-M/R(P)) showed better hemocompatibility than other samples. Endothelial cells were regulated in the direction of micropattern showing elongated ECs were closer to a healthy, athero-protective phenotype than ECs cultured in vitro without micropattern. NO and PGI(2) release were upregulated. Simultaneously the number of SMCs on Ti-PDA-M/R(P) was the smaller that of other samples, which demonstrated that the Ti-PDA-M/R(P) had property of inhibiting SMCs proliferation to a certain extent.
机译：为了调节钛（Ti）的生物材料上内皮细胞（ECS）的行为和功能，将2-甲基丙烯酰氧基乙基磷胆碱（PolymPC）和Gly-Arg-glu的聚合物的仿生微型图（脊/槽：25/25m） -ASP-VAL-TYR（GREDVY）制造。选择PMMPC（单体含有含有羧基的MPC和甲基丙烯酸（MA）），PMMPC通过氨基和羧基的缩合反应与六亚甲基二胺交联。同时，交联PMMPC（PMMPC-HD）的羧基可以与聚二胺的氨基反应，该聚二胺可以牢固地粘附在许多材料上。将Gredvy固定在多斑嗪上，但不在PMMPC-HD上，因为氨基和羧基可以与多多胺的儿茶酚和氨基反应。 IR和H-1 NMR证明PMMPC-HD已成功合成。和QCM-D（石英晶体微稳态，带耗散）和IR批准，PMMPC-HD和GREDVY可以固定在多碳胺（PDA）上。用PMMPC和GREDVY（Ti-PDA-M / R（P））修饰的微型粘连和全血粘附（Ti-PDA-M / R（P））显示出比其他样品更好的血液振动性。内皮细胞在显示细长ECS的微图示的方向上调节，比在没有微图案的体外培养的ECS的细长ECS更接近ECS的健康，热的保护表型。不上调NO和PGI（2）释放。同时，Ti-PDA-M / R（P）上的SMC数量越小，其他样品的数量越小，证明Ti-PDA-M / R（P）具有抑制SMC增殖在一定程度上的性质。

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  • 来源
    《RSC Advances》 |2018年第37期|共15页
  • 作者

    Ma Wenyong; Liu Luying; Chen Huiqing; Zhao Yuancong; Yang Ping; Huang Nan;

  • 作者单位

    Southwest Jiaotong Univ Sch Mat Sci &

    Technol Minist Educ Key Lab Adv Technol Mat Chengdu 610031 Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Technol Minist Educ Key Lab Adv Technol Mat Chengdu 610031 Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Technol Minist Educ Key Lab Adv Technol Mat Chengdu 610031 Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Technol Minist Educ Key Lab Adv Technol Mat Chengdu 610031 Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Technol Minist Educ Key Lab Adv Technol Mat Chengdu 610031 Sichuan Peoples R China;

    Southwest Jiaotong Univ Sch Mat Sci &

    Technol Minist Educ Key Lab Adv Technol Mat Chengdu 610031 Sichuan Peoples R China;

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