• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Recombinant human BMP-7 grafted poly(lactide-co-glycolide)/hydroxyapatite scaffolds via polydopamine for enhanced calvarial repair
【24h】

Recombinant human BMP-7 grafted poly(lactide-co-glycolide)/hydroxyapatite scaffolds via polydopamine for enhanced calvarial repair

机译：通过聚多巴胺重组人BMP-7嫁接的聚（丙交酯-共-乙交酯）/羟基磷灰石支架以增强颅骨修复

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Poly(lactic- co -glycolic acid) (PLGA) and hydroxyapatite (HA) are considered potential osteoinductive materials because of their biodegradability and mineralization features. However, the hydrophobicity of scaffold surfaces is less supportive of cell attachment and proliferation because of poor wettability. The mode of binding of growth factors to the scaffold also affects cell differentiation into osteoblasts. The half-life of a growth factor in vivo can be increased by binding the factor to the scaffold surface. In this work, we prepared a porous PLGA/HA scaffold grafted with recombinant human bone morphogenic protein-7 (rhBMP-7) attached via polydopamine (pDA) for bone repair. The pDA coated PLGA/HA (pDA-PLGA/HA) scaffolds were characterized by energy dispersive X-ray analysis and Fourier-transform infrared spectroscopy. The microstructure and porosity of PLGA/HA scaffolds were analyzed by scanning electron microscopy and micro-computed tomography. The release profile of rhBMP-7 grafted onto the pDA-PLGA/HA (pDA-PLGA/HA/BMP-7) scaffolds was examined for 21 days. The attachment efficiency, cell proliferation rate, alkaline phosphatase activity, calcium deposition, and osteoblast-related gene expression of bone marrow-derived stem cells to PLGA/HA, pDA-PLGA/HA, and pDA-PLGA/HA/BMP-7 scaffolds were evaluated. To assess the ability of bone repair in vivo , scaffolds were implanted into critical-sized calvarial defects created in mice, and the in vivo tissue-engineered bone was monitored by micro-computed tomography and histology. In vivo experiments revealed rapid healing of the defects treated with the pDA-PLGA/HA/BMP-7 scaffolds compared with pDA-PLGA/HA and PLGA/HA scaffolds at week 8 post-surgery. These results collectively demonstrate that the rhBMP-7-immobilized PLGA/HA scaffold via pDA is a promising candidate for calvarial repair.
机译：聚乳酸-乙醇酸（PLGA）和羟基磷灰石（HA）被认为是潜在的骨诱导材料，因为它们具有生物降解性和矿化特性。然而，由于润湿性差，支架表面的疏水性不太支持细胞附着和增殖。生长因子与支架的结合方式也影响细胞分化为成骨细胞。通过将生长因子结合至支架表面，可以增加其在体内的半衰期。在这项工作中，我们制备了多孔PLGA / HA支架，该支架移植了通过聚多巴胺（pDA）连接的重组人骨形态发生蛋白7（rhBMP-7）进行骨修复。通过能量色散X射线分析和傅里叶变换红外光谱对pDA涂层的PLGA / HA（pDA-PLGA / HA）支架进行了表征。 PLGA / HA支架的微观结构和孔隙率通过扫描电子显微镜和显微计算机断层扫描进行分析。检查移植到pDA-PLGA / HA（pDA-PLGA / HA / BMP-7）支架上的rhBMP-7的释放曲线，持续21天。骨髓干细胞对PLGA / HA，pDA-PLGA / HA和pDA-PLGA / HA / BMP-7支架的附着效率，细胞增殖速率，碱性磷酸酶活性，钙沉积和成骨细胞相关基因表达被评估。为了评估体内骨修复的能力，将支架植入小鼠体内产生的临界大小的颅骨缺损中，并通过微计算机断层扫描和组织学对体内组织工程化的骨进行监测。体内实验显示，在术后8周，与pDA-PLGA / HA和PLGA / HA支架相比，用pDA-PLGA / HA / BMP-7支架治疗的缺损得以快速治愈。这些结果共同证明，通过pDA固定化rhBMP-7的PLGA / HA支架是颅盖修复的有希望的候选者。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号