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Insight into the effect of quinic acid on biofilm formed by Staphylococcus aureus

机译：了解奎宁酸对金黄色葡萄球菌形成的生物膜的影响

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摘要

The biofilm formation of Staphylococcus aureus on food contact surfaces is the main risk of food contamination. In the present study, we firstly investigated the inhibitory effect of quinic acid (QA) on biofilm formed by S. aureus . Crystal violet staining assay and microscopy analysis clearly showed that QA at sub-MIC concentrations was able to significantly reduce the biofilm biomass and cause a collapse on biofilm architecture. Meanwhile, fibrinogen binding assay showed that QA had obviously effect on the S. aureus bacteria adhesion. XTT reduction assay and confocal laser scanning microscopic images revealed that QA significantly decreased metabolic activity and viability of biofilm cells. In addition, qRT-PCR analysis explored the potential inhibitory mechanism of QA against biofilm formation, which indicated that QA significantly repressed the gene sarA and activated the gene agrA . Moreover, QA exhibited a highly ability to reduce the number of sessile S. aureus cells adhered on the stainless steel. So, it was suggested that QA could be used as a promising antibiofilm agent to control biofilm formation of S. aureus .
机译：食物接触表面上金黄色葡萄球菌的生物膜形成是食物污染的主要风险。在本研究中，我们首先研究了奎尼酸（QA）对金黄色葡萄球菌形成的生物膜的抑制作用。结晶紫染色测定法和显微镜分析清楚地表明，低于MIC浓度的QA能够显着减少生物膜生物量并导致生物膜结构崩溃。同时，纤维蛋白原结合试验表明，质量保证对金黄色葡萄球菌的粘附有明显的影响。 XTT还原测定和共聚焦激光扫描显微图像显示，QA显着降低了生物膜细胞的代谢活性和活力。此外，qRT-PCR分析探索了QA抑制生物膜形成的潜在机制，这表明QA显着抑制了sarA基因并激活了agrA基因。此外，QA表现出很高的减少粘附在不锈钢上的无核金黄色葡萄球菌细胞数量的能力。因此，有人建议将QA用作控制金黄色葡萄球菌生物膜形成的有前途的抗生物膜剂。

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