首页> 外文期刊>RSC Advances >SNP discovery and functional annotation in the Panax japonicus var. major transcriptome
【24h】

SNP discovery and functional annotation in the Panax japonicus var. major transcriptome

机译:人参中的SNP发现和功能注释。主要转录组

获取原文
           

摘要

Due to the lack of a Panax japonicus var. major reference genome, we assembled a reference transcriptome from P. japonicus C. A. Mey transcriptome sequencing data, and 203?283 unigenes were obtained. In this study, with the assistance from the Trinity, Bowtie2 and SAMtools softwares, 218?465 single nucleotide polymorphisms (SNPs) were identified by mapping the Illumina sequences to the reference transcriptome. The SNP forms included 126?262 transformations and 92?203 transversions. A large number of SNP loci were associated with triterpenoid saponin synthesis: 54 SNPs were associated with cytochrome P450, one with glycosyl transferase and 94 with the biosynthesis of the triterpenoid saponin backbone.
机译:由于缺少人参变种。在主要参考基因组中,我们从日本对虾C. A. Mey转录组测序数据中组装了一个参考转录组,并获得203?283个单基因。在这项研究中,借助于Trinity,Bowtie2和SAMtools软件,通过将Illumina序列映射到参考转录组确定了218-465个单核苷酸多态性(SNP)。 SNP形式包括126→262转化和92→203转化。大量的SNP位点与三萜皂苷的合成有关:54个SNP与细胞色素P450相关,一个与糖基转移酶相关,94个与三萜皂苷骨架的生物合成相关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号